-transparent.png){kind=logo}
The extended use of Fish with centromeric probes in many cytogenetic laboratories is often impaired by the cost of this technique. Polymerase Chain Reaction (PCR) constitutes a simple way to generate and label such centromeric probes at low cost. Two types of human DNA source can be used: 1--Somatic hybrid cell lines containing a unique human chromosome. The specific amplification of the human subset of alphoid DNA is realised with a primer pair specific for the consensus region of human alpha satellite sequence. 2--Total Human DNA. This time, a primer pair specific for the alpha satellite DNA of the chromosome of interest must be designed. These probes, labelled during the PCR reaction by direct incorporation of modified dUTP, are actually widely used in our laboratory, alone or mixed with other probes (chromosome painting or locus specific probes).
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
January 1994,
Methods in molecular biology (Clifton, N.J.),
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
December 1990,
Molecular and cellular probes,
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
February 1994,
Analytical biochemistry,
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
January 1996,
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology,
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
January 1992,
Sexually transmitted diseases,
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
November 1996,
Cancer genetics and cytogenetics,
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
March 1998,
The European respiratory journal. Supplement,
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
September 1990,
Proceedings of the National Academy of Sciences of the United States of America,
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
January 1996,
Scanning microscopy. Supplement,
J M Dupont, and
A Lebbar, and
O Dupuy, and
N Frydman, and
D Letessier, and
P Auvinet, and
D Rabineau
May 1993,
Biotechnic & histochemistry : official publication of the Biological Stain Commission,
