Cryopreservation of immature and mature human oocytes. 2002

J D Wininger, and H I Kort
Reproductive Biology Associates, Atlanta, Georgia 30342, USA.

Cryopreservation of oocytes facilitates the long-term storage of oocytes for patients in danger of losing ovarian function. It also alleviates many of the ethical concerns associated with embryo cryopreservation. Problems associated with metaphase II oocyte cryopreservation include zona pellucida hardening and spindle damage. The cryopreservation of germinal vesicle-stage oocytes has been undertaken as a means of circumventing the problem of spindle damage in mature oocytes. One of the main disadvantages of immature oocyte cryopreservation is the fact that in vitro maturation is required post-thaw. The majority of live births from oocyte cryopreservation have involved the use of 1,2-propanediol and slow freezing protocols. Various methods have been used in an attempt to improve survival rates. These include vitrification and use of novel cryopreservatives. Future areas of concentration should include in vitro maturation, vitrification, and alternate cryopreservatives.

UI MeSH Term Description Entries
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D003451 Cryoprotective Agents Substances that provide protection against the harmful effects of freezing temperatures. Cryoprotective Agent,Cryoprotective Effect,Cryoprotective Effects,Agent, Cryoprotective,Agents, Cryoprotective,Effect, Cryoprotective,Effects, Cryoprotective
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015925 Cryopreservation Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens. Cryofixation,Cryonic Suspension,Cryonic Suspensions,Suspension, Cryonic
D019946 Propylene Glycol A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations. 1,2-Propanediol,Propan-1,2-Diol,Propylene Glycol Monohydrate,Propylene Glycol Sodium Salt,Propylene Glycol, (+-)-Isomer,Propylene Glycol, (R)-Isomer,Propylene Glycol, (S)-Isomer,1,2 Propanediol,Glycol, Propylene,Monohydrate, Propylene Glycol
D020554 Sperm Injections, Intracytoplasmic An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE). ICSI,Injections, Sperm, Intracytoplasmic,Intracytoplasmic Sperm Injections,Injection, Intracytoplasmic Sperm,Injections, Intracytoplasmic Sperm,Intracytoplasmic Sperm Injection,Sperm Injection, Intracytoplasmic

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