-transparent.png){kind=logo}
The lysosomal form (L form) of beta-glucuronidase was purified 6,500-fold from the liver of C57BL/6J mice with high yield. Purified enzyme was homogeneous as judged by polyacrylamide gel electrophoresis in the presence or absence of sodium dodetcyl sulfate. The microsomal forms of beta-glucuronidase were spontaneously converted to the L form. The purified L form is a tetramer of molecular weight of 280,000 to 300,000, composedd of four identical subunits of 75,000 molecular weight. The enzyme contains a high content of arginine and glutamic acid and a very low content of sulfur-containing amino acids. Approximately 7% of the enzyme molecule is compose of carbohydrate. Sugars in the L form are glucosamine, mannose, galactose, and glucose. Sialic acid and fucose are absent in the enzyme.
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
October 1970,
Biochimica et biophysica acta,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
June 1953,
The Journal of biological chemistry,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
March 1981,
The Biochemical journal,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
June 1975,
The Journal of biological chemistry,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
January 1962,
The Journal of biological chemistry,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
December 1951,
The Biochemical journal,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
September 1966,
Archives of biochemistry and biophysics,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
July 1965,
The Journal of biological chemistry,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
April 1953,
The Biochemical journal,
S Tomino, and
K Paigen, and
D R Tulsiani, and
O Touster
November 1976,
European journal of biochemistry,
