Synergistic effects of p-fluorophenylalanine and supra-optimal temperatures on cell division in Tetrahymena pyriformis. 1979

D N Wheatley, and E Zeuthen, and L Rasmussen

The combined effects of sub-optimal (20 degrees) or supra-optimal (31 degrees C and 34 degrees C) temperatures and the amino acid analogue p-fluorophenylalanine on preparations for cell division in Tetrahymena pyriformis were analysed. A supra-optimal temperature of 31 degrees produced a marked exaggeration of the division-delaying capacity of the analogue. The analogue also sensitises cells when combined with the normal heat shock temperature of 34 degrees, which indicates that division proteins continue to be synthesized at this temperature but cannot be effectively utilized by the cell. These results support the model that preparations involve interactions between and formation of assemblies of proteins on a strict time schedule shortly before division.

UI MeSH Term Description Entries
D010135 p-Fluorophenylalanine 3-(p-Fluorophenyl)-alanine. para-Fluorophenylalanine,4-Fluorophenylalanine,p Fluorophenylalanine,4 Fluorophenylalanine,Fluorophenylalanine, p,para Fluorophenylalanine
D010649 Phenylalanine An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE. Endorphenyl,L-Phenylalanine,Phenylalanine, L-Isomer,L-Isomer Phenylalanine,Phenylalanine, L Isomer
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D013769 Tetrahymena pyriformis A species of ciliate protozoa used extensively in genetic research. Tetrahymena pyriformi,pyriformi, Tetrahymena
D014176 Protein Biosynthesis The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS. Genetic Translation,Peptide Biosynthesis, Ribosomal,Protein Translation,Translation, Genetic,Protein Biosynthesis, Ribosomal,Protein Synthesis, Ribosomal,Ribosomal Peptide Biosynthesis,mRNA Translation,Biosynthesis, Protein,Biosynthesis, Ribosomal Peptide,Biosynthesis, Ribosomal Protein,Genetic Translations,Ribosomal Protein Biosynthesis,Ribosomal Protein Synthesis,Synthesis, Ribosomal Protein,Translation, Protein,Translation, mRNA,mRNA Translations

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