Molecular cloning and characterization of the mouse reticulon 3 cDNA. 2002

Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
Department of Virology, Kurume University School of Medicine, Fukuoka, Japan.

cDNA of mouse reticulon 3 (mRTN3) was cloned. The cloned cDNA is 1745 bp long and contains an open reading frame of 237 amino acids for a 30 kDa protein. The gene was mapped at band B of chromosome 19 by FISH. Two altematively spliced transcripts, 3.4 and 2.3 kb, of mRTN3 were found by Northem blot analysis. Both transcripts were expressed in many tissues and embryos and the highest expression of the 3.4 kb-transcript was observed in the brain, especially in neurons. The expression of 30 kDa-mRTN3 protein was also greatest in the brain. Both N and C-termini of mRTN3 faced the cytosol, indicating that they may recruit other proteins to the endoplasmic reticulum.

UI MeSH Term Description Entries
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008815 Mice, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation. Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004721 Endoplasmic Reticulum A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed) Ergastoplasm,Reticulum, Endoplasmic
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
November 1996, Molecular and cellular endocrinology,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
August 1989, Gene,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
November 1993, Biochimica et biophysica acta,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
May 2001, Biochimica et biophysica acta,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
February 1998, Gene,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
September 1994, Genomics,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
July 1998, Biochimica et biophysica acta,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
May 1998, British journal of pharmacology,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
November 1986, Proceedings of the National Academy of Sciences of the United States of America,
Nobuyuki Hamada, and Jun Iwahashi, and Kentaro Suzuki, and Hidenao Ogi, and Takahito Kashiwagi, and Koyu Hara, and Michiko Toyoda, and Tatsuo Yamada, and Tetsuya Toyoda
March 1999, Biological & pharmaceutical bulletin,
Copied contents to your clipboard!