-transparent.png){kind=logo}
The effects of Con A, WGA, Zymosan A on macrophage cytosolic pH and outflow of lysosomal content through exocytosis were studied with SNAFL-calcein and FITC-Dextran on ACAS570. The results showed all three ligands could induce macrophage cytosolic acidification in about 10 min and kept at the same level hereafter; outflow of lysosomal fluorescent probe through exocytosis appeared in 15-20 min. In resting conditions, macrophage lysosomes mainly distributed in cell center; after stimulated for 15 min by three ligands, the number of lysosomes increased in membrane periphery, in 25-30 min lysosomes moved back toward cell center. We proposed that ligands induced lysosomal pH rises was a basic factor for outflow of lysosomal content through exocytosis, cytosolic acidification inhibited receptor-mediated endocytosis. Cytosolic acidification and outflow of lysosomal content through exocytosis were the results of cellular self-regulation and self-protection during receptor-mediated endocytosis.
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
January 1987,
Advances in experimental medicine and biology,
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
February 1984,
Biochemical and biophysical research communications,
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
June 1988,
The American journal of physiology,
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
February 1983,
Journal of cellular physiology,
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
January 2021,
Cell reports,
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
November 1990,
Neuron,
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
March 2007,
Apoptosis : an international journal on programmed cell death,
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
January 2010,
Advances in pharmacology (San Diego, Calif.),
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
April 1999,
The Plant cell,
G H Lei, and
W Zhang, and
J C Wu, and
Y J Piao, and
Y Y Bao, and
H Huang
April 1983,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
