Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays. 2002

Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
Laboratory of Reproductive Medicine, Nanjing Medical University, 210029, P.R.China. shajh@njmu.edu.cn

We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1-4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models.

UI MeSH Term Description Entries
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D013091 Spermatogenesis The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA. Spermatocytogenesis,Spermiogenesis
D013737 Testis The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS. Testicles,Testes,Testicle
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
January 2006, Cellular & molecular biology letters,
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
July 2002, Genome biology,
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
February 2023, Marine biotechnology (New York, N.Y.),
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
September 2017, Die Naturwissenschaften,
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
November 2000, Endocrinology,
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
January 2003, Journal of andrology,
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
January 2007, European journal of histochemistry : EJH,
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
May 1996, Nature,
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
July 2014, Yi chuan = Hereditas,
Jiahao Sha, and Zuomin Zhou, and Jianmin Li, and Lanlan Yin, and Huanmin Yang, and Gengxi Hu, and Ming Luo, and Hsiao Chang Chan, and Kaiya Zhou, and
May 2004, Zygote (Cambridge, England),
Copied contents to your clipboard!