Biomaterial Database

[Fluorescence study of X and Y chromatin in frozen sections]. 1975

K Gyula

Chinacrine fluorescence of the Y chromatin in histological sections is a useful tool for determination of nuclear sex. Nuclei of fibroblasts and smooth muscle cells seems to be most suitable for this investigation. Tissue specimens should be fixed for 5 minutes in hot formalin. Frequency of the Y chromatin in frozen sections of male origin was found to be 38--70 per cent, in paraffin embedded sections 28--44 per cent. It seems likely, that paraffin decreases chinacrin binding capacity of the Y chromatin. Frequency of the Barr-body in tissue specimens of female origin was found to be similar both in frozen and paraffin embedded sections. For the investigation of X and Y chromatin in tissue sections the technique of frozen sections should be used.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008856	Microscopy, Fluorescence	Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.	Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D003106	Colon	The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.	Appendix Epiploica,Taenia Coli,Omental Appendices,Omental Appendix,Appendices, Omental,Appendix, Omental
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001065	Appendix	A worm-like blind tube extension from the CECUM.	Vermiform Appendix,Appendix, Vermiform
D001706	Biopsy	Removal and pathologic examination of specimens from the living body.	Biopsies
D012728	Sex Chromatin	In the interphase nucleus, a condensed mass of chromatin representing an inactivated X chromosome. Each X CHROMOSOME, in excess of one, forms sex chromatin (Barr body) in the mammalian nucleus. (from King & Stansfield, A Dictionary of Genetics, 4th ed)	Barr Bodies,Chromatin, Sex,Bodies, Barr,Chromatins, Sex,Sex Chromatins
D012730	Sex Chromosomes	The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed)	Gonosomes,Chromosome, Sex,Chromosomes, Sex,Gonosome,Sex Chromosome
D012867	Skin	The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.