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Crystallization and preliminary X-ray diffraction analysis of glutamate dehydrogenase from an aerobic hyperthermophilic archaeon, Aeropyrum pernix K1. 2002

Mohammad W Bhuiya, and Hideaki Tsuge, and Haruhiko Sakuraba, and Kazunari Yoneda, and Nobuhiko Katunuma, and Toshihisa Ohshima
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minamijosanjimacho, Tokushima 770-8506, Japan.

Glutamate dehydrogenase from an aerobic hyperthermophilic archaeon, Aeropyrum pernix K1, was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol (PEG) 400 as the precipitant. The crystals belong to the hexagonal space group P6(3), with unit-cell parameters a = b = 98.9, c = 394.8 A, alpha = beta = 90, gamma = 120 degrees. The asymmetric unit contained one hexamer of the enzyme, giving a crystal volume per enzyme mass (V(M)) of 1.98 A(3) Da(-1) and a solvent content of 37.3%. The X-ray diffraction data were collected to a resolution of 3.0 A at the BL6B beamline in the Photon Factory with an overall R(sym) of 13.8% and a completeness of 87.1%.

UI MeSH Term Description Entries
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D018086 Glutamate Dehydrogenase (NADP+) An enzyme that catalyzes the conversion of L-glutamate, H2O, and NADP+ to 2-oxoglutarate, NH3, and NADPH. (From Enzyme Nomenclature, 1992) EC 1.4.1.4. NADP+-Dependent Glutamate Dehydrogenase,NADP-Dependent Glutamate Dehydrogenase,NADP-Specific Glutamate Dehydrogenase,NADPH-Dependent Glutamate Dehydrogenase,Dehydrogenase, NADP+-Dependent Glutamate,Dehydrogenase, NADP-Dependent Glutamate,Dehydrogenase, NADP-Specific Glutamate,Dehydrogenase, NADPH-Dependent Glutamate,Glutamate Dehydrogenase, NADP+-Dependent,Glutamate Dehydrogenase, NADP-Dependent,Glutamate Dehydrogenase, NADP-Specific,Glutamate Dehydrogenase, NADPH-Dependent,NADP Dependent Glutamate Dehydrogenase,NADP Specific Glutamate Dehydrogenase,NADP+ Dependent Glutamate Dehydrogenase,NADPH Dependent Glutamate Dehydrogenase
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies
D019620 Desulfurococcaceae A family of archaea, in the order DESULFUROCOCCALES, consisting of anaerobic cocci which utilize peptides, proteins or carbohydrates facultatively by sulfur respiration or fermentation. There are eight genera: AEROPYRUM, Desulfurococcus, Ignicoccus, Staphylothermus, Stetteria, Sulfophoboccus, Thermodiscus, and Thermosphaera. (From Bergey's Manual of Systematic Bacteriology, 2d ed) Desulfurococcus,Ignicoccus,Staphylothermus,Stetteria,Sulfophobococcus,Thermodiscus,Thermosphaera

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