[Immunodiagnostic tests for bilharziasis. IV. Demonstration of antibody in blood and serum eluates collected on filter paper discs (author's transl)]. 1975

S Oelerich, and D W Büttner, and G Dietz, and H Muhl

Serum and blood samples dried on filter paper discs and normal sera were collected from 142 Liberians with proven schistosomiasis and 25 Liberians without schistosomiasis. These samples were tested by the indirect hemagglutination test (IHA) employing lyophilized sheep erythrocytes sensitized with either cercarial or adult Schistosoma mansoni antigens. The following results were obtained: Out of the 142 sera collected from the schistosomiasis patients 130 (91.5%) reacted with the cercarial antigen and only 89 (62.6%) reacted with the adult S. mansoni antigen. None of the 25 sera from persons free of schistosomiasis reacted with either antigen. The sensitized lyophilized erythrocytes showed no loss of antibody activity after laboratory storage at 4 degrees C for 4 months which was interrupted twice for 16 hours each during shipment. Filter paper serum and blood samples were stored at -25 degrees C up to 84 days with 16 hours interruption during shipment. 77.6% of the eluates from blood showed the same antibody titer levels as in normal serum; 10% showed lower antibody titers and 11.5% had negative results compared with normal serum. Results obtained with eluates from dried serum were somewhat better. An additional storage of the dried blood specimens for ten days at room temperature resulted in a rise of the proportion of negative titers from 11.5% to 34.1%. This loss of antibody activity was found in two thirds of the samples with low antibody titers. The results indicate that blood and serum samples dried on filter paper platelets should be stored at low temperatures (-25 degrees C) to preserve the antibody response.

UI MeSH Term Description Entries
D010209 Paper Thin sheets made from wood pulp and other fibrous substances, used for writing, drawing, printing, image duplication or wrapping. Papers
D001793 Blood Preservation The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Blood Preservations,Preservation, Blood,Preservations, Blood
D001800 Blood Specimen Collection The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc. Blood Specimen Collections,Collection, Blood Specimen,Collections, Blood Specimen,Specimen Collection, Blood,Specimen Collections, Blood
D003080 Cold Temperature An absence of warmth or heat or a temperature notably below an accustomed norm. Cold,Cold Temperatures,Temperature, Cold,Temperatures, Cold
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D005374 Filtration A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Filtrations
D006386 Hemagglutination Tests Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed) Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).

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