Natural genetic recombination between co-circulating heterotypic enteroviruses. 2002

Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
Cantacuzino Institute, Splaiul Independentei 103, Bucharest, R-70.100 Romania1.

Natural recombination in poliovirus is a frequent phenomenon. In practice, whenever different genotypes have the opportunity to infect the same individual, a high proportion of viruses with recombinant genomes are excreted. To determine whether enteroviruses other than poliovirus can naturally produce viable virions with recombinant genomes, we studied the molecular features of two distant regions of the viral genomes - the VP1 coding region and the 3D polymerase coding region - of the echovirus serotypes associated with a large outbreak of aseptic meningitis. Nucleotide sequences of nine epidemic strains [belonging to echovirus serotypes 4 (E4), 7 (E7) and 30 (E30)] in the two genomic regions (300 nt of VP1 and 520 nt of 3D polymerase) were compared to prototype and field strains, and phylogenetic trees were generated from alignments. In the VP1 region, each of the three epidemic serotypes clustered with the homotypic prototype strain, whereas in the 3D polymerase region, E7 and E30 grouped as a single cluster, distant from the two corresponding prototype strains. This suggests that one of these two E7 and E30 strains has evolved through recombination with the other or that both have acquired the 3D polymerase coding region from a common ancestor. Our results suggest that such genetic recombinations between different echovirus serotypes are possible when multiple epidemic strains are circulating simultaneously.

UI MeSH Term Description Entries
D008582 Meningitis, Aseptic A syndrome characterized by headache, neck stiffness, low grade fever, and CSF lymphocytic pleocytosis in the absence of an acute bacterial pathogen. Viral meningitis is the most frequent cause although MYCOPLASMA INFECTIONS; RICKETTSIA INFECTIONS; diagnostic or therapeutic procedures; NEOPLASTIC PROCESSES; septic perimeningeal foci; and other conditions may result in this syndrome. (From Adams et al., Principles of Neurology, 6th ed, p745) Aseptic Meningitis
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D011995 Recombination, Genetic Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses. Genetic Recombination,Recombination,Genetic Recombinations,Recombinations,Recombinations, Genetic
D004196 Disease Outbreaks Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS. Outbreaks,Infectious Disease Outbreaks,Disease Outbreak,Disease Outbreak, Infectious,Disease Outbreaks, Infectious,Infectious Disease Outbreak,Outbreak, Disease,Outbreak, Infectious Disease,Outbreaks, Disease,Outbreaks, Infectious Disease
D004769 Enterovirus Infections Diseases caused by ENTEROVIRUS. Infections, Enterovirus,Enterovirus Infection,Infection, Enterovirus
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012321 DNA-Directed RNA Polymerases Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992). DNA-Dependent RNA Polymerases,RNA Polymerases,Transcriptases,DNA-Directed RNA Polymerase,RNA Polymerase,Transcriptase,DNA Dependent RNA Polymerases,DNA Directed RNA Polymerase,DNA Directed RNA Polymerases,Polymerase, DNA-Directed RNA,Polymerase, RNA,Polymerases, DNA-Dependent RNA,Polymerases, DNA-Directed RNA,Polymerases, RNA,RNA Polymerase, DNA-Directed,RNA Polymerases, DNA-Dependent,RNA Polymerases, DNA-Directed
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D012383 Romania A country in southeastern Europe, bordering the Black Sea, between Bulgaria and Ukraine. The capital is Bucharest. Rumania,Roumania

Related Publications

Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
October 2003, Journal of virology,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
March 1980, Virology,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
January 2008, Journal of virology,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
February 2014, The Journal of general virology,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
October 1999, Journal of virology,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
January 1987, Voprosy virusologii,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
January 2011, PloS one,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
March 2014, The Journal of general virology,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
February 1989, The Journal of general virology,
Gabriela Oprisan, and Mariana Combiescu, and Sophie Guillot, and Valerie Caro, and Andrei Combiescu, and Francis Delpeyroux, and Radu Crainic
May 1955, Journal of cellular physiology. Supplement,
Copied contents to your clipboard!