This study investigated the effect of spatial accuracy demands on movement organization by analyzing the amplitude of the agonist and antagonist muscle activities emerging during horizontal elbow-flexion movements toward spatial targets of varying difficulties. 8 subjects performed elbow-flexion movements toward targets of 3 sizes, located at 2 distances, as rapidly and accurately as possible. For each movement, the elbow angles and the activities of biceps brachii, brachioradialis, and lateral and long heads of triceps brachii were measured. Analysis on the kinematic variables indicated that final elbow angle and peak velocity decreased with increasing index of difficulty of the task in both movement-amplitude conditions. However, movement time increased with increasing index of difficulty. The amplitude of agonist and antagonist muscle activities measured for 100 msec. before movement initiation was also shown to decrease with increasing index of difficulty. Agonist and antagonist muscle activities measured during acceleration phase displayed similar patterns with those of premovement. These results suggest that the task difficulty affects movement organization, and the control system decreases the amplitude of agonist and antagonist muscle activities with an increase in the index of difficulty to enhance the controllability of the limb.