Effect of medium composition on the susceptibility of oral streptococci to mercuric chloride. 2002

Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
Department of Microbiology, Eastman Dental Institute, University College London, 256 Grays Inn Road, London WC1X 8LD, UK.

Although there is considerable interest in identifying mercury-resistant bacteria, no standardized assay exists for this purpose. In this study, the effect of the composition of the medium on the susceptibility of oral streptococci to HgCl(2) was investigated. The minimum inhibitory concentration (MIC) of HgCl(2) for 52 streptococcal strains and the reproducibility of MIC values for Hg-sensitive and Hg-resistant strains was determined with 11 different media. Addition of blood increased the MIC values, and some media (tryptone soya agar, with or without blood) could not discriminate between Hg-sensitive and Hg-resistant strains. The proportion of streptococci that appeared to be resistant to Hg was very high (>70%) on some media (mitis-salivarius, tryptone soya, Columbia), but not on others (Mueller-Hinton, Brain Heart Infusion, Isosensitest). The MICs of the control strains varied considerably on different testing occasions for tryptone soya agar (with and without blood), Isosensitest agar, and Columbia agar (with blood). Mueller-Hinton (without blood) appeared to be the most suitable medium for isolating Hg-resistant oral streptococci.

UI MeSH Term Description Entries
D008627 Mercuric Chloride Mercury chloride (HgCl2). A highly toxic compound that volatizes slightly at ordinary temperature and appreciably at 100 degrees C. It is corrosive to mucous membranes and used as a topical antiseptic and disinfectant. Mercury Dichloride,Corrosive Sublimate,HgCl2,Mercuric Perchloride,Mercury Bichloride,Mercury Perchloride,Sublimate,Bichloride, Mercury,Chloride, Mercuric,Dichloride, Mercury,Perchloride, Mercuric,Perchloride, Mercury,Sublimate, Corrosive
D008826 Microbial Sensitivity Tests Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses). Bacterial Sensitivity Tests,Drug Sensitivity Assay, Microbial,Minimum Inhibitory Concentration,Antibacterial Susceptibility Breakpoint Determination,Antibiogram,Antimicrobial Susceptibility Breakpoint Determination,Bacterial Sensitivity Test,Breakpoint Determination, Antibacterial Susceptibility,Breakpoint Determination, Antimicrobial Susceptibility,Fungal Drug Sensitivity Tests,Fungus Drug Sensitivity Tests,Sensitivity Test, Bacterial,Sensitivity Tests, Bacterial,Test, Bacterial Sensitivity,Tests, Bacterial Sensitivity,Viral Drug Sensitivity Tests,Virus Drug Sensitivity Tests,Antibiograms,Concentration, Minimum Inhibitory,Concentrations, Minimum Inhibitory,Inhibitory Concentration, Minimum,Inhibitory Concentrations, Minimum,Microbial Sensitivity Test,Minimum Inhibitory Concentrations,Sensitivity Test, Microbial,Sensitivity Tests, Microbial,Test, Microbial Sensitivity,Tests, Microbial Sensitivity
D009055 Mouth The oval-shaped oral cavity located at the apex of the digestive tract and consisting of two parts: the vestibule and the oral cavity proper. Oral Cavity,Cavitas Oris,Cavitas oris propria,Mouth Cavity Proper,Oral Cavity Proper,Vestibule Oris,Vestibule of the Mouth,Cavity, Oral
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000362 Agar A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013211 Staphylococcus aureus Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D013291 Streptococcus A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.

Related Publications

Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
January 2019, Biocontrol science,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
April 1983, Scandinavian journal of dental research,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
March 1919, The Journal of medical research,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
January 1976, Journal of dental research,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
January 1972, Caries research,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
May 1984, Gigiena truda i professional'nye zabolevaniia,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
May 1954, Biulleten' eksperimental'noi biologii i meditsiny,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
August 1979, Experimental and molecular pathology,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
April 2008, Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine,
Rachel Pike, and Paul Stapleton, and Victoria Lucas, and Graham Roberts, and Robin Rowbury, and Hillary Richards, and Peter Mullany, and Michael Wilson
May 1987, The Journal of antimicrobial chemotherapy,
Copied contents to your clipboard!