Vinculin and meta-vinculin in fast and slow rat skeletal muscle before and after hindlimb suspension. 2002

Angèle Chopard, and Françoise Pons, and Jean-François Marini
Laboratoire de Physiologie Cellulaire et Moléculaire des Systèmes Intégrés, CNRS, Unité Mixte de Recherche 6548, Faculté des Sciences, Parc Valrose, 06108 Nice Cedex 2, France. achopard@unice.fr

The role of vinculin and meta-vinculin, the major components of costameres, was explored by analysing quantitatively the expression of these proteins in a slow-twitch (soleus) and in a fast-twitch (extensor digitorum longus, EDL) skeletal muscle under control conditions and after a reduced functional demand. Meta-vinculin, previously observed essentially in smooth and cardiac muscle, was also present in EDL, in the same amount as vinculin. The soleus contained exclusively vinculin, the amount of which exceeded the sum of vinculin and meta-vinculin in the EDL. After 3 weeks hypokinesia (hindlimb suspension), the vinculin content of the soleus was unchanged but after 6 weeks it had increased by 20% and, moreover, there was de novo expression of meta-vinculin. In EDL, the changes in vinculin and meta-vinculin after 3 weeks were opposite (+26% and -20% respectively). After 6 weeks the increase in vinculin was even larger (+33%) while meta-vinculin had returned to control levels. The marked expression of meta-vinculin in the atrophied soleus suggests a shift in the soleus towards the fast muscle profile with respect to cytoskeletal characteristics. On the other hand, the ability of slow muscle to maintain posture and to generate force for long periods would necessitate cytoskeletal reinforcement.

UI MeSH Term Description Entries
D009929 Organ Size The measurement of an organ in volume, mass, or heaviness. Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015992 Body Mass Index An indicator of body density as determined by the relationship of BODY WEIGHT to BODY HEIGHT. BMI Quetelet Index,Quetelet's Index,Index, Body Mass,Index, Quetelet,Quetelets Index
D016474 Weight-Bearing The physical state of supporting an applied load. This often refers to the weight-bearing bones or joints that support the body's weight, especially those in the spine, hip, knee, and foot. Load-Bearing,Axial Loading,Loadbearing,Weightbearing,Axial Loadings,Load Bearing,Weight Bearing
D016596 Vinculin A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10. alpha-Vinculin,beta-Vinculin,Isovinculin,alpha Vinculin,beta Vinculin
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D018482 Muscle, Skeletal A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles. Anterior Tibial Muscle,Gastrocnemius Muscle,Muscle, Voluntary,Plantaris Muscle,Skeletal Muscle,Soleus Muscle,Muscle, Anterior Tibial,Muscle, Gastrocnemius,Muscle, Plantaris,Muscle, Soleus,Muscles, Skeletal,Muscles, Voluntary,Skeletal Muscles,Tibial Muscle, Anterior,Voluntary Muscle,Voluntary Muscles
D018656 Muscle Fibers, Fast-Twitch Skeletal muscle fibers characterized by their expression of the Type II MYOSIN HEAVY CHAIN isoforms which have high ATPase activity and effect several other functional properties - shortening velocity, power output, rate of tension redevelopment. Several fast types have been identified. Muscle Fibers, Intermediate,Muscle Fibers, Type II,Muscle Fibers, White,Fast-Twitch Muscle Fiber,Fast-Twitch Muscle Fibers,Fiber, Fast-Twitch Muscle,Fiber, Intermediate Muscle,Fiber, White Muscle,Fibers, Fast-Twitch Muscle,Fibers, Intermediate Muscle,Fibers, White Muscle,Intermediate Muscle Fiber,Intermediate Muscle Fibers,Muscle Fiber, Fast-Twitch,Muscle Fiber, Intermediate,Muscle Fiber, White,Muscle Fibers, Fast Twitch,White Muscle Fiber,White Muscle Fibers
D018657 Muscle Fibers, Slow-Twitch Skeletal muscle fibers characterized by their expression of the Type I MYOSIN HEAVY CHAIN isoforms which have low ATPase activity and effect several other functional properties - shortening velocity, power output, rate of tension redevelopment. Muscle Fibers, Red,Muscle Fibers, Type I,Fiber, Red Muscle,Fiber, Slow-Twitch Muscle,Fibers, Red Muscle,Fibers, Slow-Twitch Muscle,Muscle Fiber, Red,Muscle Fiber, Slow-Twitch,Muscle Fibers, Slow Twitch,Red Muscle Fiber,Red Muscle Fibers,Slow-Twitch Muscle Fiber,Slow-Twitch Muscle Fibers

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