Effect of diet on Shiga toxin-producing Escherichia coli (STEC) growth and survival in rumen and abomasum fluids. 2002

Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
Laboratoire de Microbiologie, INRA, St-Genès-Champanelle, France.

The gastrointestinal tract of ruminants is the main reservoir for Shiga toxin-producing Escherichia coli (STEC) strains, potentially pathogenic for humans. We used for the first timerumen fluid in which no exogenous carbon source or other supplement was added to compare acid resistance and growth of STEC in physiological physico-chemical conditions. We showed that acidic conditions resulting from the combination of high volatile fatty acid concentration and moderately acidic pH did not alter the survival of STEC, and that human non-O157:H7 STEC isolates were able to persist in the rumen contents in spite of acid stress, low oxygen availability and nutrient deprivation, in the same manner as bovine STEC isolates do. Furthermore, our results support the hypothesis that a grain-rich diet may induce mechanisms of STEC acid resistance in the rumen that allow STEC survival in the abomasum.

UI MeSH Term Description Entries
D010100 Oxygen An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration. Dioxygen,Oxygen-16,Oxygen 16
D004032 Diet Regular course of eating and drinking adopted by a person or animal. Diets
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005232 Fatty Acids, Volatile Short-chain fatty acids of up to six carbon atoms in length. They are the major end products of microbial fermentation in the ruminant digestive tract and have also been implicated in the causation of neurological diseases in humans. Fatty Acids, Short-Chain,Short-Chain Fatty Acid,Volatile Fatty Acid,Acid, Short-Chain Fatty,Acid, Volatile Fatty,Fatty Acid, Short-Chain,Fatty Acid, Volatile,Fatty Acids, Short Chain,Short Chain Fatty Acid,Short-Chain Fatty Acids,Volatile Fatty Acids
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000018 Abomasum The fourth stomach of ruminating animals. It is also called the "true" stomach. It is an elongated pear-shaped sac lying on the floor of the abdomen, on the right-hand side, and roughly between the seventh and twelfth ribs. It leads to the beginning of the small intestine. (From Black's Veterinary Dictionary, 17th ed) Abomasums
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012417 Rumen The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed) Rumens
D012703 Serotyping Process of determining and distinguishing species of bacteria or viruses based on antigens they share. Serotypings
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli

Related Publications

Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
March 2010, Clinics in laboratory medicine,
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
January 2003, Methods in molecular biology (Clifton, N.J.),
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
November 2021, Antibiotics (Basel, Switzerland),
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
January 2022, Frontiers in cellular and infection microbiology,
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
March 2015, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology],
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
September 2016, Communicable diseases intelligence quarterly report,
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
April 1999, Berliner und Munchener tierarztliche Wochenschrift,
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
December 2013, Pathology,
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
August 1999, Letters in applied microbiology,
Karima Boukhors, and Nathalie Pradel, and Jean-Pierre Girardeau, and Valérie Livrelli, and Ahmed Mohamed Ou Saïd, and Michel Contrepois, and Christine Martin
January 2014, Frontiers in microbiology,
Copied contents to your clipboard!