Biomaterial Database

Cholinesterase measurements with an automated kit. 2002

Georgino H Oliveira, and John D Henderson, and Barry W Wilson

Department of Animal Science, University of California, Davis, California 95616, USA.

BACKGROUND The Test-mate kit determines acetylcholinesterase (AChE, EC 3.1.1.7) and hemoglobin content of a drop of blood, displaying enzyme activities normalized to 25 degrees C. Previous models produced inconsistent results at different temperatures. This report focuses on the current model, ChE 400, and two instruments of a previous OP model. METHODS AChE activities were determined by the Ellman assay, using the three kits and a 96-well microplate reader. Temperatures ranged from 10 to 37 degrees C. Fetal bovine serum was the source of AChE. RESULTS Normalized activities decreased below 20 degrees C in the ChE model and below 25 degrees C in the OP models. Activities of the same serum sample differed between the three Test-mate kits, ranging from 1.03 to 1.49 micromoles/min/ml. Percent errors were greater than with the microplate reader at all temperatures. CONCLUSIONS Neither we nor the manufacturer recommend the current Test-mate model for fieldwork. Nevertheless, there have been field measurements with Test-Mate kits, and we recommend that an enzyme activity standard be run in parallel with their use.

UI	MeSH Term	Description	Entries
D010575	Pesticides	Chemicals used to destroy pests of any sort. The concept includes fungicides (FUNGICIDES, INDUSTRIAL); INSECTICIDES; RODENTICIDES; etc.	Pesticide
D004796	Clinical Enzyme Tests	Analyses for a specific enzyme activity, or of the level of a specific enzyme that is used to assess health and disease risk, for early detection of disease or disease prediction, diagnosis, and change in disease status.	Enzyme Tests, Clinical,Clinical Enzyme Test,Enzyme Test, Clinical,Test, Clinical Enzyme,Tests, Clinical Enzyme
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000110	Acetylcholinesterase	An enzyme that catalyzes the hydrolysis of ACETYLCHOLINE to CHOLINE and acetate. In the CNS, this enzyme plays a role in the function of peripheral neuromuscular junctions. EC 3.1.1.7.	Acetylcholine Hydrolase,Acetylthiocholinesterase,Hydrolase, Acetylcholine
D000382	Agricultural Workers' Diseases	Diseases in persons engaged in cultivating and tilling soil, growing plants, harvesting crops, raising livestock, or otherwise engaged in husbandry and farming. The diseases are not restricted to farmers in the sense of those who perform conventional farm chores: the heading applies also to those engaged in the individual activities named above, as in those only gathering harvest or in those only dusting crops.	Agricultural Worker Disease,Agricultural Worker's Disease,Agricultural Worker Diseases,Agricultural Worker's Diseases,Agricultural Workers Disease,Agricultural Workers Diseases,Agricultural Workers' Disease,Disease, Agricultural Worker,Disease, Agricultural Worker's,Disease, Agricultural Workers',Diseases, Agricultural Worker,Diseases, Agricultural Worker's,Diseases, Agricultural Workers',Worker Disease, Agricultural,Worker Diseases, Agricultural,Worker's Disease, Agricultural,Worker's Diseases, Agricultural,Workers' Disease, Agricultural,Workers' Diseases, Agricultural
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D015203	Reproducibility of Results	The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.	Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016273	Occupational Exposure	The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation.	Exposure, Occupational,Exposures, Occupational,Occupational Exposures