-transparent.png){kind=logo}
Yeast ribosomal RNA was hydrolyzed to its constituent nucleosides with the aid of snake venom and bacterial alkaline phosphatase. Lyophilized hydrolysate was labeled with radioactive 5-hydroxyuridine and applied to partition chromatography. It was found that some components of rRNA are held on the column and can be eluted with water. Eighty-nine percent of the label, a large portion of cytidine, and several unidentified compounds were found in the water wash. The direct application of the wash concentrate to ascending paper chromatography in saturated butanol-H2O resulted in the separation of three distinct UV-absorbing bands. Further resolution and characterization of one band of unidentified material revealed the presence of an additional nucleoside. On the basis of chromatographic and electrophoretic behavior and UV-absorption spectra, it was tentatively identified as 5-hydroxymethyluridine.
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
January 1965,
The Journal of biological chemistry,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
June 1966,
Archives of biochemistry and biophysics,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
July 1992,
The Journal of biological chemistry,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
January 1971,
Biochimica et biophysica acta,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
January 1969,
Journal of molecular biology,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
January 1972,
Physiological chemistry and physics,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
March 1964,
Biochimica et biophysica acta,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
August 1987,
FEBS letters,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
April 1969,
Biochimica et biophysica acta,
A W Lis, and
R K Mclaughlin, and
D I Mclaughlin
June 2009,
BMB reports,
