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1. Purine nucleoside phosphorylase (purine nucleoside:orthophosphate ribosyltransferase, E.C. 2.4.2.1) from liver of cattle, Bos taurus, was purified to homogeneity. Some properties of the enzymes from three different bovine tissues were compared and discussed. 2. The enzyme has a molecular weight of 83,000, a sedimentation coefficient of 5.3 S, a Stokes' radius of 3.71 nm, a frictional ratio of 1.30 and a subunit molecular weight of 30,000. 3. Optimal pH for xanthosine degradation is around 5.5, whereas a broad pH activity profile for inosine degradation was observed between 5.0 and 7.5. Lineweaver-Burk plots curved downward at high concentrations of substrates, inosine, phosphate and arsenate.
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
January 1991,
Advances in experimental medicine and biology,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
January 1978,
Methods in enzymology,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
November 1992,
Biochimica et biophysica acta,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
January 1984,
Revue d'elevage et de medecine veterinaire des pays tropicaux,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
April 1971,
Biochimica et biophysica acta,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
January 1978,
Methods in enzymology,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
January 1980,
The International journal of biochemistry,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
January 2013,
Biomeditsinskaia khimiia,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
June 1979,
Nihon rinsho. Japanese journal of clinical medicine,
Z Ikezawa, and
T Nishino, and
K Murakami, and
K Tsushima
June 1976,
Archives of biochemistry and biophysics,
