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Adenosine triphosphatase (ATPase) was studied in tissue homogenates and subcellular fractions derived from human cadaver kidneys maintained in an organ preservation unit for transplantation. The activity of ouabain-sensitive ATPase was highest in the medulla, intermediate in the cortex and lowest in the papilla. The cortical enzyme activity diminished with time during maintenance perfusion of the kidneys. Similar concentrations of K+, Na+, Mg++, ATP and MgATP were required for half-maximal rates of ouabain-sensitive ATPase activity from the cortex or the medulla. The sensitivity of the enzyme to ouabain from both parts of the kidney was similar. K+ antagonized inhibition of the enzyme by ouabain. Chlormerodrin, mersalyl, mercaptomerin and ethacrynic acid were inhibitors of the enzyme.
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
August 1974,
The Journal of biological chemistry,
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
September 1973,
British journal of haematology,
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
June 1975,
Cryobiology,
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
January 1976,
Biochemical Society transactions,
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
March 1981,
Biokhimiia (Moscow, Russia),
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
June 1978,
Biokhimiia (Moscow, Russia),
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
October 1976,
Analytical biochemistry,
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
September 1970,
FEBS letters,
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
May 1968,
The Journal of biological chemistry,
B R Nechay, and
J A Nelson, and
R R Contreras, and
H E Sarles, and
A R Remmers, and
G A Beathard, and
J C Fish, and
J D Lindley, and
J M Brady, and
M J Lerman
March 1964,
Experimental eye research,
