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Sphingosylphosphorylcholine-induced contraction of feline ileal smooth muscle cells is mediated by Galphai3 protein and MAPK. 2002

Tai Lee, and Jin Kim, and Uy Sohn
Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul 156-756, Republic of Korea.

We studied the mechanism of sphingosylphosphorylcholine (SPC)-induced contraction in feline ileal smooth muscle cells. Western blotting revealed that G protein subtypes of Galpha(i1), Galpha(i3) and Galpha(o) existed in feline ileum. Galpha(i3) antibody penetration into permeabilized cells decreased SPC-induced contraction. In addition, incubation of [35S]guanosine 5'-O-(3-thiotriphosphate) ([35S]GTPgammaS) with membrane fraction increased its binding to Galpha(i3) subtype after SPC treatment, suggesting that the signalling pathways invoked by SPC were mediated by Galpha(i3) protein. MAPK kinase (MEK) inhibitor PD98059 blocked the contraction significantly, but p38 mitogen-activated protein kinase (MAPK) inhibitor SB202190 did not. Chelerythrine and neomycin also inhibited the contraction. However, cotreatment of PD98059 and chelerythrine showed no significant difference. Phosphorylation of p44/42 MAPK was increased by SPC treatment, which was reversed by pretreatment of inhibitors of signalling molecules that decreased SPC-induced contraction previously. The same result was obtained in the assay of MAPK activity.

UI MeSH Term Description Entries
D007082 Ileum The distal and narrowest portion of the SMALL INTESTINE, between the JEJUNUM and the ILEOCECAL VALVE of the LARGE INTESTINE.
D008297 Male Males
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009130 Muscle, Smooth Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed) Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D010767 Phosphorylcholine Calcium and magnesium salts used therapeutically in hepatobiliary dysfunction. Choline Chloride Dihydrogen Phosphate,Choline Phosphate Chloride,Phosphorylcholine Chloride,Choline Phosphate,Phosphocholine,Chloride, Choline Phosphate,Chloride, Phosphorylcholine,Phosphate Chloride, Choline,Phosphate, Choline
D002415 Cats The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801) Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013110 Sphingosine An amino alcohol with a long unsaturated hydrocarbon chain. Sphingosine and its derivative sphinganine are the major bases of the sphingolipids in mammals. (Dorland, 28th ed) 4-Sphingenine,4 Sphingenine
D019206 GTP-Binding Protein alpha Subunits, Gi-Go A family of heterotrimeric GTP-binding protein alpha subunits that were originally identified by their ability to inhibit ADENYLYL CYCLASES. Members of this family can couple to beta and gamma G-protein subunits that activate POTASSIUM CHANNELS. The Gi-Go part of the name is also spelled Gi/Go. G-Protein, Gi-Go alpha Family,G-Protein, Inhibitory Gi,Gi, Inhibitory G-Protein,G Protein Go,G Protein Subunit alphaoA,G Protein Subunit alphaoC,G(o) Protein,G(o1)alpha,G-Protein, Gi Subunit,G-Protein, Gi-Go Subunits,G-Protein, Go Subunit,G-Protein, Go1 Subunit,G-Protein, Inhibitory Go,GTP-Binding Protein alpha Subunit, Gi,GTP-Binding Protein alpha Subunit, Go,GTP-Binding Protein alpha Subunit, Go1,GTP-Binding Protein alpha Subunit, GoA,GTP-Binding Protein alpha Subunit, GoC,Gi alpha Subunit,Gi-Alpha Protein,Gi-Go G-Proteins,Go Alpha Subunit,Guanine Nucleotide-Binding Protein Go,Ni Protein,Alpha Subunit, Go,G Protein, Gi Go Subunits,G Protein, Gi Go alpha Family,G Protein, Gi Subunit,G Protein, Go Subunit,G Protein, Go1 Subunit,G Protein, Inhibitory Gi,G Protein, Inhibitory Go,G-Proteins, Gi-Go,GTP Binding Protein alpha Subunit, Gi,GTP Binding Protein alpha Subunit, Go,GTP Binding Protein alpha Subunit, Go1,GTP Binding Protein alpha Subunit, GoA,GTP Binding Protein alpha Subunit, GoC,GTP Binding Protein alpha Subunits, Gi Go,Gi Alpha Protein,Gi G-Protein, Inhibitory,Gi Go G Proteins,Gi Subunit G-Protein,Gi, Inhibitory G Protein,Gi-Go Subunits G-Protein,Go G-Protein, Inhibitory,Go Subunit G-Protein,Go, G Protein,Go1 Subunit G-Protein,Guanine Nucleotide Binding Protein Go,Inhibitory G-Protein Gi,Inhibitory Gi G-Protein,Inhibitory Go G-Protein,Subunit G-Protein, Gi,Subunit G-Protein, Go,Subunit G-Protein, Go1,Subunit, Gi alpha,Subunit, Go Alpha,Subunits G-Protein, Gi-Go,alpha Subunit, Gi
D020928 Mitogen-Activated Protein Kinases A superfamily of PROTEIN SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES). Mitogen Activated Protein Kinase,Mitogen-Activated Protein Kinase,Kinase, Mitogen-Activated Protein,Kinases, Mitogen-Activated Protein,Mitogen Activated Protein Kinases,Protein Kinase, Mitogen-Activated,Protein Kinases, Mitogen-Activated

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