Nucleic acid content and nucleotide composition of DNA in leptospirae. 1975

G I Ezhov, and V S Kiktenko, and T T Berezov

The contents of DNA and RNA were studied in 9 strains of leptospirae and the composition of nitrogen bases of DNA in 20 strains of pathogenic and saprophytic leptospirae. It has been found that leptospirae contain a high per cent ration of nucleic acids. According to the nucleotide composition, the family of Leptospira as a whole belongs to the AT-type. According to overall guanine and cytosine contents, the investigated strains of pathogenic leptospirae fall into 3 groups and differ from the seprophytic strains. With respect to the limited number of the investigated strains, the DNA nucleotide composition of leptospirae can be used as a supplementary biochemical criterion in the classification of leptospirae.

UI MeSH Term Description Entries
D007919 Leptospira A genus of aerobic, helical spirochetes, some species of which are pathogenic, others free-living or saprophytic.
D009711 Nucleotides The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed) Nucleotide
D002854 Chromatography, Paper An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase). Paper Chromatography,Chromatographies, Paper,Paper Chromatographies
D003596 Cytosine A pyrimidine base that is a fundamental unit of nucleic acids.
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D006147 Guanine
D012329 RNA, Bacterial Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis. Bacterial RNA
D013053 Spectrophotometry The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.

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