Increased superoxide radicals generation from alveolar macrophages in immature guinea-pigs. 2002

Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
Department of Pediatriacs, Gunma University School of Medicine, Maebashi, Gunma, 371-8511, Japan.

To study the effect of maturation on abilities of superoxide radicals (O-2) generation in the airways, we compared stimuli-induced O-2 generation by alveolar macrophages in immature (aged 10+/-2 days) and adult (aged 90+/-2 days) guinea-pigs. The production of O-2 was assayed by chemiluminescence method, using a Cypridina luciferin analog as a highly sensitive and specific probe for O-2. Whereas no significant difference in cell components of bronchoalveolar lavage fluid was observed between immature and adult animals, O-2 generation induced by stimulation of alveolar macrophages was greater in immature than in adult animals, with significant differences observed after platelet-activating factor (100 nM) or phorbol myristate acetate (0.5 micro g/ml). The results suggest that alveolar macrophages from immature animals are far more potent O-2 generators than the same cells of adult animals.

UI MeSH Term Description Entries
D008163 Luminescent Measurements Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE. Bioluminescence Measurements,Bioluminescent Assays,Bioluminescent Measurements,Chemiluminescence Measurements,Chemiluminescent Assays,Chemiluminescent Measurements,Chemoluminescence Measurements,Luminescence Measurements,Luminescent Assays,Luminescent Techniques,Phosphorescence Measurements,Phosphorescent Assays,Phosphorescent Measurements,Assay, Bioluminescent,Assay, Chemiluminescent,Assay, Luminescent,Assay, Phosphorescent,Assays, Bioluminescent,Assays, Chemiluminescent,Assays, Luminescent,Assays, Phosphorescent,Bioluminescence Measurement,Bioluminescent Assay,Bioluminescent Measurement,Chemiluminescence Measurement,Chemiluminescent Assay,Chemiluminescent Measurement,Chemoluminescence Measurement,Luminescence Measurement,Luminescent Assay,Luminescent Measurement,Luminescent Technique,Measurement, Bioluminescence,Measurement, Bioluminescent,Measurement, Chemiluminescence,Measurement, Chemiluminescent,Measurement, Chemoluminescence,Measurement, Luminescence,Measurement, Luminescent,Measurement, Phosphorescence,Measurement, Phosphorescent,Measurements, Bioluminescence,Measurements, Bioluminescent,Measurements, Chemiluminescence,Measurements, Chemiluminescent,Measurements, Chemoluminescence,Measurements, Luminescence,Measurements, Luminescent,Measurements, Phosphorescence,Measurements, Phosphorescent,Phosphorescence Measurement,Phosphorescent Assay,Phosphorescent Measurement,Technique, Luminescent,Techniques, Luminescent
D008297 Male Males
D011650 Pulmonary Alveoli Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place. Alveoli, Pulmonary,Alveolus, Pulmonary,Pulmonary Alveolus
D001992 Bronchoalveolar Lavage Fluid Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung. Alveolar Lavage Fluid,Bronchial Lavage Fluid,Lung Lavage Fluid,Bronchial Alveolar Lavage Fluid,Lavage Fluid, Bronchial,Lavage Fluid, Lung,Pulmonary Lavage Fluid,Alveolar Lavage Fluids,Bronchial Lavage Fluids,Bronchoalveolar Lavage Fluids,Lavage Fluid, Alveolar,Lavage Fluid, Bronchoalveolar,Lavage Fluid, Pulmonary,Lavage Fluids, Alveolar,Lavage Fluids, Bronchial,Lavage Fluids, Bronchoalveolar,Lavage Fluids, Lung,Lavage Fluids, Pulmonary,Lung Lavage Fluids,Pulmonary Lavage Fluids
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000367 Age Factors Age as a constituent element or influence contributing to the production of a result. It may be applicable to the cause or the effect of a circumstance. It is used with human or animal concepts but should be differentiated from AGING, a physiological process, and TIME FACTORS which refers only to the passage of time. Age Reporting,Age Factor,Factor, Age,Factors, Age
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013481 Superoxides Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides. Superoxide Radical,Superoxide,Superoxide Anion
D016676 Macrophages, Alveolar Round, granular, mononuclear phagocytes found in the alveoli of the lungs. They ingest small inhaled particles resulting in degradation and presentation of the antigen to immunocompetent cells. Alveolar Macrophages,Macrophages, Pulmonary,Pulmonary Macrophages,Macrophage, Pulmonary,Pulmonary Macrophage,Alveolar Macrophage,Macrophage, Alveolar

Related Publications

Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
January 1986, Comparative biochemistry and physiology. B, Comparative biochemistry,
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
February 1992, Journal of lipid mediators,
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
December 1976, The Journal of nutrition,
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
February 1985, The New England journal of medicine,
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
January 1990, Allergologia et immunopathologia,
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
February 1988, Agents and actions,
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
July 1984, Journal of leukocyte biology,
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
July 2008, Tuberculosis (Edinburgh, Scotland),
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
October 1990, Biulleten' eksperimental'noi biologii i meditsiny,
Masahiko Kato, and Kenichi Tokuyama, and Hisanori Minakami, and Akira Nagai, and Kunihisa Kozawa, and Hideo Goto, and Akihiro Morikawa, and Hirokazu Kimura
March 1987, Cancer research,
Copied contents to your clipboard!