A novel method for preparing histology slides to integrate the teaching of gross and microscopic anatomy. 2002

Judy A Provo-Klimek, and Deryl L Troyer
Department of Anatomy and Physiology, Kansas State University College of Veterinary Medicine, 228 Coles Hall, Manhattan, KS 66506, USA. provo@vet.ksu.edu

The authors have previously reported the development of a novel technique for sampling and preparing tissue slides for routine microscopic examination, without the use of a microtome. Termed "RAMP" (Rapid Adhesive Mediated Procedure), this simple, albeit somewhat crude, technique holds promise as a method that can be used in the field by veterinary practitioners for rapid microscopic evaluations to obtain early preliminary estimates of the nature of a mass or lesion. We incorporated the use of this method into a gross anatomy course in an attempt to gauge its utility for novices in tissue sampling and histology slide preparation. By having each group of students take a tissue sample from their cadaver, the activity simulated an actual necropsy situation in which practitioners in the field might use the technique. Because students were able to follow their specimen from sampling to microscopic examination, the activity provided a valuable integration of their learning of gross and microscopic anatomy. We conducted an evaluation of the process and the resulting slides with two successive classes of students. We conclude that the RAMP method is reasonably successful in the hands of individuals not trained in tissue preparation; was well received by the students as a valuable learning tool; and could potentially yield useful histological information for practicing veterinarians. Limitations of the method are also discussed.

UI MeSH Term Description Entries
D004520 Education, Veterinary Use for general articles concerning veterinary medical education. Veterinary Education,Educations, Veterinary,Veterinary Educations
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000715 Anatomy A branch of biology dealing with the structure of organisms. Anatomies
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013048 Specimen Handling Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation. Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D013663 Teaching A formal and organized process of transmitting knowledge to a person or group. Teaching Method,Training Activity,Training Technique,Academic Training,Educational Technics,Educational Techniques,Pedagogy,Teaching Methods,Technics, Educational,Techniques, Educational,Training Activities,Training Technics,Training Techniques,Activities, Training,Activity, Training,Educational Technic,Educational Technique,Method, Teaching,Methods, Teaching,Pedagogies,Technic, Educational,Technic, Training,Technics, Training,Technique, Educational,Technique, Training,Techniques, Training,Training Technic,Training, Academic
D019411 Clinical Laboratory Techniques Techniques used to carry out clinical investigative procedures in the diagnosis and therapy of disease. Clinical Laboratory Test,Clinical Laboratory Testing,Clinical Laboratory Diagnoses,Clinical Laboratory Testings,Clinical Laboratory Tests,Diagnoses and Laboratory Examinations,Diagnosis, Laboratory,Laboratory Diagnosis,Laboratory Examinations and Diagnoses,Laboratory Techniques, Clinical,Clinical Laboratory Technique,Diagnose, Clinical Laboratory,Laboratory Diagnoses,Laboratory Technique, Clinical,Laboratory Test, Clinical,Laboratory Testing, Clinical,Technique, Clinical Laboratory,Test, Clinical Laboratory,Testing, Clinical Laboratory

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