Biomaterial Database

Homocysteine-induced augmentation of spontaneous contraction of isolated guinea pig myometrium. 2002

Ahmet Ayar, and Niyazi Tug, and Husnu Celik, and Mete Ozcan, and Oguz Ozcelik

Firat (Euphrates) University, Faculty of Medicine, Department of Pharmacology, Elazig, Turkey. aayar@firat.edu.tr

Recent studies have suggested association between the elevated homocysteine levels and pregnancy complications. The aim of this study was to investigate the effects of homocysteine on spontaneous contractions of myometrium isolated from pregnant guinea pig. Full-thickness myometrial strips were obtained from late-pregnant guinea pigs following decapitation, and suspended in an organ bath which was filled with physiological saline solution (pH 7.4), maintained at 37 degrees C and continuously bubbled with 95% O2 and 5% CO2. After the observation of spontaneous contractions under one gram of resting tension, homocysteine (0.2, 0.5 and 1 mM) was added to the organ bath and effects of this agent on frequency and amplitude of contractions were evaluated in 20-min periods. One-way analysis of variance was used for statistical analysis of the data. Homocysteine caused increase in both frequency and amplitude of spontaneous contractions in a dose-dependent manner. The results from this in vitro study indicate that cardiovascular risk factor homocysteine has contracting pharmacological effects on guinea pig myometrium.

UI	MeSH Term	Description	Entries
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005260	Female		Females
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006710	Homocysteine	A thiol-containing amino acid formed by a demethylation of METHIONINE.	2-amino-4-mercaptobutyric acid,Homocysteine, L-Isomer,2 amino 4 mercaptobutyric acid,Homocysteine, L Isomer,L-Isomer Homocysteine
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014590	Uterine Contraction	Contraction of the UTERINE MUSCLE.	Myometrial Contraction,Contraction, Myometrial,Contraction, Uterine,Contractions, Myometrial,Contractions, Uterine,Myometrial Contractions,Uterine Contractions
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In