Biomaterial Database

Identification of conidial-enriched transcripts in Aspergillus nidulans using suppression subtractive hybridization. 2002

Nir Osherov, and John Mathew, and Angela Romans, and Gregory S May

Division of Pathology and Laboratory Medicine, Box 54, M D Anderson Cancer Center, The University of Texas, 1515 Holcombe Blvd, Houston, TX 77030, USA.

We have isolated and sequence-identified 12 genes whose transcripts are significantly enriched in Aspergillus nidulans conidia. To identify these genes, we used the method of suppressive subtraction hybridization (SSH). One of the 12 genes is similar to plant thaumatin-like proteins that have antifungal properties. Four genes encode metabolic enzymes crucial in the synthesis of glucose, carbohydrates, nucleic acid, and amino acid precursors. The rest are of unknown function. We have analyzed the pattern of expression of the 12 conidial-enriched transcripts in wild-type and mutant strains of A. nidulans blocked at different stages of conidial development. Our results indicate that the conidial-enriched transcripts can be divided into four classes based on their expression pattern in the wild-type and mutant strains. Study of the genes identified in this report may enhance our understanding of the process of conidial formation and germination.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D002241	Carbohydrates	A class of organic compounds composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n. The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES.	Carbohydrate
D005800	Genes, Fungal	The functional hereditary units of FUNGI.	Fungal Genes,Fungal Gene,Gene, Fungal
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006824	Hybridization, Genetic	The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.	Crossbreeding,Hybridization, Intraspecies,Crossbreedings,Genetic Hybridization,Genetic Hybridizations,Hybridizations, Genetic,Hybridizations, Intraspecies,Intraspecies Hybridization,Intraspecies Hybridizations
D001233	Aspergillus nidulans	A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.	Aspergillus nidulellus,Emericella nidulans
D013172	Spores, Fungal	Reproductive bodies produced by fungi.	Conidia,Fungal Spores,Conidium,Fungal Spore,Spore, Fungal
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription
D015152	Blotting, Northern	Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.	Northern Blotting,Blot, Northern,Northern Blot,Blots, Northern,Blottings, Northern,Northern Blots,Northern Blottings
D018076	DNA, Complementary	Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.	Complementary DNA,cDNA,cDNA Probes,Probes, cDNA