Biomaterial Database

The influence of the mycotoxins deoxynivalenol and zearalenol on in vitro maturation of pig oocytes and in vitro culture of pig zygotes. 2002

H Alm, and T Greising, and K-P Brüssow, and H Torner, and U Tiemann

Department of Reproductive Biology, Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany. alm@fbn-dummerstorf.de

The aim of the present study was to investigate the influence of specific toxins on in vitro maturation and embryo culture. alpha- and beta-zearalenol were tested at increasing levels from 3.75 to 90 microM and deoxynivalenol from 0.94 to 7.5 microM in order to evaluate the effect on in vitro maturation rate of porcine cumulus-oocyte complexes. Furthermore, the influence of alpha-zearalenol (3.75-30 microM) was appraised on the developmental competence of in vivo-derived zygotes during 5 days of in vitro culture. All three substances affected maturation and degeneration rates in a dose-dependent manner, but to different extents. Significant differences were obtained at a concentration of 7.5 microM alpha-zearalenol and higher. beta-zearalenol negatively affected the process of oocyte development beginning at a concentration of 30.0 microM (P<0.05). Deoxynivalenol had significant influence on oocyte maturation at a concentration of 1.88 microM (31.4 vs 79.3% for control). Differences in embryonic development in vitro were observed at a concentration of 15 microM alpha-zearalenol (P<0.05). These data demonstrate a negative effect of alpha-zearalenol on embryonic development of zygotes, and a compound-specific, dose-dependent negative effect of the three substances on meiotic progression of porcine oocytes.

UI	MeSH Term	Description	Entries
D009865	Oocytes	Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).	Ovocytes,Oocyte,Ovocyte
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005314	Embryonic and Fetal Development	Morphological and physiological development of EMBRYOS or FETUSES.	Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D014255	Trichothecenes	Usually 12,13-epoxytrichothecenes, produced by Fusaria, Stachybotrys, Trichoderma and other fungi, and some higher plants. They may contaminate food or feed grains, induce emesis and hemorrhage in lungs and brain, and damage bone marrow due to protein and DNA synthesis inhibition.	Epoxytrichothecenes,Trichothecene Epoxides,Epoxides, Trichothecene
D015029	Zeranol	A non-steroidal estrogen analog.	Dihydroxyundecyl Resorcylic Acid Lactone,Zearalanol,P-1496,P1496,Ralgro,Ralone,Xeranol,Zearanol,P 1496
D015053	Zygote	The fertilized OVUM resulting from the fusion of a male and a female gamete.	Fertilized Ovum,Ovum, Fertilized,Fertilized Egg,Egg, Fertilized,Eggs, Fertilized,Fertilized Eggs,Zygotes
D018929	Cell Culture Techniques	Methods for maintaining or growing CELLS in vitro.	Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell