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Structure of the O-specific polysaccharide of Providencia alcalifaciens O16 containing N-acetylmuramic acid. 2002
Nina A Kocharova, and
George V Zatonsky, and
Olga V Bystrova, and
Andrzej Ziolkowski, and
Marianna Wykrota, and
Aleksander S Shashkov, and
Yuriy A Knirel, and
Antoni Rozalski
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, GSP-1, Moscow, Russia.
The O-specific polysaccharide of Providencia alcalifaciens O16 was obtained by mild-acid degradation of the lipopolysaccharide and studied by chemical methods and NMR spectroscopy, including 2D 1H,(1)H COSY, TOCSY, NOESY, and 1H,(13)C HSQC experiments. It was found that the polysaccharide contains N-acetylmuramic acid, which was isolated by solvolysis with trifluoromethanesulfonic acid and identified by the specific optical rotation and NMR spectroscopy. The following structure of the trisaccharide repeating-unit of the polysaccharide was established:
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