Biomaterial Database

[Effects of respiratory syncytial virus infection on T-helper cytokines expression in Balb/c mice sensitized with ovalbumin]. 2002

Yi Zou, and Jun Xu, and Nanshan Zhong

Guangzhou Institute of Respiratory Disease, Guangzhou 510120, China.

OBJECTIVE To determine the effects of RSV infection on the development of lung inflammation and the expressions of T-helper cell related cytokines in Balb/c mice pre-sensitized with ovalbumin. METHODS Forty mice were randomly divided into four groups: (1) the control group: nonsensitized, noninfected; (2) the RSV group: nonsensitized, infected; (3) the OVA group: sensitized, noninfected; and (4) the OVA + RSV group: sensitized, infected (RSV 6.5 x 10(6) PFU nasal drip). Bronchoalveolar lavage (BAL) was performed in six mice from each group five days after RSV nasal inoculation. Cells in the BAL were counted and classified and the supernatants of the BALF were used for detection of IL-4 and IFN-gamma. Four mice in each group were sacrificed for lung RNA extraction, and IL-4, IL-5 and IFN-gamma mRNA levels were determined by RT-PCR. RESULTS (1) Total BAL cells from the RSV group [(14.5 +/- 5.4) x 10(6)/ml] and the OVA + RSV group [(16.8 +/- 4.9) x 10(6)/ml] were significantly higher than those in the control group [(7.7 +/- 2.4) x 10(6)/ml] and the OVA group [(9.0 +/- 2.5) x 10(6)/ml] (all P < 0.05), with more lymphocytes in the RSV group (0.63 +/- 0.05) and the OVA + RSV group (0.77 +/- 0.09) than the control group (0.28 +/- 0.05) (P < 0.05) and the OVA group (0.36 +/- 0.03) (P < 0.05). More eosinophils were found in the OVA + RSV group (0.0690 +/- 0.0100) than in the RSV group (0.0090 +/- 0.0050), the OVA group (0.0100 +/- 0.0040) and the control (0.0030 +/- 0.0010) (all P < 0.05). (2) Pulmonary inflammation in the OVA + RSV group and the RSV group was more severe than that in the OVA group and the control group. More lymphocyte infiltration was found in the OVA + RSV and the RSV groups. More eosinophils were found in the OVA + RSV group, but not in the other groups. (3) Expression of IL-4, IL-5, and IFN-gamma mRNA: IFN-gamma mRNA expression (1.05 +/- 0.12) was higher in the RSV group, than in the control (0.00 +/- 0.00). There were only minimal expression of IL-5 mRNA and no expression of IL-4 mRNA, indicating a Th1-like cytokine response. Expressions of IL-4 mRNA and IL-5 mRNA were significantly higher in the OVA + RSV group than in the other groups, but the IFN-gamma mRNA expression was less than that in the RSV group, indicating a Th2-like cytokine response. (4) IFN-gamma activation in BALF from the RSV group [(33.8 +/- 1.1) pg/ml] was significantly higher than that in the other groups (P < 0.05), while IL-4 showed no significant change as compared to the control group. The level of IL-4 in the OVA + RSV group [(24.0 +/- 1.5) pg/ml] was significantly higher than that in all the other groups (P < 0.05). CONCLUSIONS RSV infection in OVA-sensitized Balb/c mice caused a different immune response from that caused by RSV infection in nonsensitized mice. RSV infection alone resulted in a Th1-like cytokine response, while infection after OVA sensitization resulted in a Th2-like response.

UI	MeSH Term	Description	Entries
D007371	Interferon-gamma	The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.	Interferon Type II,Interferon, Immune,gamma-Interferon,Interferon, gamma,Type II Interferon,Immune Interferon,Interferon, Type II
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D010047	Ovalbumin	An albumin obtained from the white of eggs. It is a member of the serpin superfamily.	Serpin B14
D001992	Bronchoalveolar Lavage Fluid	Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.	Alveolar Lavage Fluid,Bronchial Lavage Fluid,Lung Lavage Fluid,Bronchial Alveolar Lavage Fluid,Lavage Fluid, Bronchial,Lavage Fluid, Lung,Pulmonary Lavage Fluid,Alveolar Lavage Fluids,Bronchial Lavage Fluids,Bronchoalveolar Lavage Fluids,Lavage Fluid, Alveolar,Lavage Fluid, Bronchoalveolar,Lavage Fluid, Pulmonary,Lavage Fluids, Alveolar,Lavage Fluids, Bronchial,Lavage Fluids, Bronchoalveolar,Lavage Fluids, Lung,Lavage Fluids, Pulmonary,Lung Lavage Fluids,Pulmonary Lavage Fluids
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012333	RNA, Messenger	RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.	Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D015847	Interleukin-4	A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.	B-Cell Growth Factor-I,B-Cell Stimulatory Factor-1,Binetrakin,IL-4,Mast Cell Growth Factor-2,B Cell Stimulatory Factor-1,B-Cell Growth Factor-1,B-Cell Proliferating Factor,B-Cell Stimulating Factor-1,B-Cell Stimulatory Factor 1,BCGF-1,BSF-1,IL4,MCGF-2,B Cell Growth Factor 1,B Cell Growth Factor I,B Cell Proliferating Factor,B Cell Stimulating Factor 1,B Cell Stimulatory Factor 1,Interleukin 4,Mast Cell Growth Factor 2
D015848	Interleukin-5	A cytokine that promotes differentiation and activation of EOSINOPHILS. It also triggers activated B-LYMPHOCYTES to differentiate into IMMUNOGLOBULIN-secreting cells.	B-Cell Growth Factor-II,Eosinophil Differentiation Factor,IL-5,T-Cell Replacing Factor,BCGF-II,Differentiation Factor, Eosinophil,IL5,T-Cell-Replacing Factor,B Cell Growth Factor II,Interleukin 5,Replacing Factor, T-Cell,T Cell Replacing Factor
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus