BIOMAT Database Logo BIOMAT Database Logo

Radiation hybrid mapping of five muscarinic acetylcholine receptor subtype genes in Rattus norvegicus. 2002

Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, 9200 W. Wisconsin Ave., Milwaukee, WI 53226, USA.

Acetylcholine is the main neurotransmitter of the vestibular efferent system and a wide variety of muscarinic and nicotinic acetylcholine receptors are expressed in the vestibular periphery. The role of these receptors and in particular the role of muscarinic acetylcholine receptors in the physiology of the vestibular neuroepithelium is not understood. Congenic and consomic rats are a convenient way to investigate the involvement of candidate genes in the manifestation of defined traits. To use congenic or consomic rats to elucidate the roles of these receptors in vestibular physiology or pathology the chromosomal location of the genes encoding these receptors has to be determined. Using radiation hybrid (RH) mapping and a rat RH map server (www.rgd.mcw.edu/RHMAP SERVER/), we determined the chromosomal locations of the muscarinic acetylcholine receptor genes in the rat (Rattus norvegicus). The m1-m5 muscarinic subtypes mapped to the following chromosomes: Chrm1, chromosome 1; Chrm2, chromosome 4; Chrm3, chromosome 17; Chrm4, chromosome 3; and Chrm5, chromosome 3. With the chromosomal location for each of these muscarinic subtypes known, it is now possible to develop congenic and consomic strains of rats that can be used to study the functions of each of these subtypes.

UI MeSH Term Description Entries
D011976 Receptors, Muscarinic One of the two major classes of cholinergic receptors. Muscarinic receptors were originally defined by their preference for MUSCARINE over NICOTINE. There are several subtypes (usually M1, M2, M3....) that are characterized by their cellular actions, pharmacology, and molecular biology. Muscarinic Acetylcholine Receptors,Muscarinic Receptors,Muscarinic Acetylcholine Receptor,Muscarinic Receptor,Acetylcholine Receptor, Muscarinic,Acetylcholine Receptors, Muscarinic,Receptor, Muscarinic,Receptor, Muscarinic Acetylcholine,Receptors, Muscarinic Acetylcholine
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D020033 Protein Isoforms Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING. Isoform,Isoforms,Protein Isoform,Protein Splice Variant,Splice Variants, Protein,Protein Splice Variants,Isoform, Protein,Isoforms, Protein,Splice Variant, Protein,Variant, Protein Splice,Variants, Protein Splice
D021101 Radiation Hybrid Mapping A method for ordering genetic loci along CHROMOSOMES. The method involves fusing irradiated donor cells with host cells from another species. Following cell fusion, fragments of DNA from the irradiated cells become integrated into the chromosomes of the host cells. Molecular probing of DNA obtained from the fused cells is used to determine if two or more genetic loci are located within the same fragment of donor cell DNA. Radiation Hybrid Maps,Radiation Hybrids,Hybrid Maps, Radiation,Hybrid, Radiation,Hybrids, Radiation,Map, Radiation Hybrid,Mapping, Radiation Hybrid,Radiation Hybrid,Radiation Hybrid Map

Related Publications

Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
Mapping of five subtype genes for muscarinic acetylcholine receptor to mouse chromosomes.
February 1999, Genes & genetic systems,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
Structural basis of muscarinic acetylcholine receptor subtype diversity.
February 1988, Trends in pharmacological sciences,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
Functional role of muscarinic acetylcholine receptor subtype diversity.
January 1988, Cold Spring Harbor symposia on quantitative biology,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
Polymorphisms in human muscarinic receptor subtype genes.
January 2012, Handbook of experimental pharmacology,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
Structural Features of Iperoxo-BQCA Muscarinic Acetylcholine Receptor Hybrid Ligands Determining Subtype Selectivity and Efficacy.
January 2022, ACS chemical neuroscience,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
On homology modeling of the M₂ muscarinic acetylcholine receptor subtype.
June 2013, Journal of computer-aided molecular design,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
[Trichinellosis in Rattus norvegicus and Rattus rattus].
July 1957, Ceskoslovenska epidemiologie, mikrobiologie, imunologie,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
Acetylcholine synthesis and muscarinic receptor subtype mRNA expression in T-lymphocytes.
January 1998, Life sciences,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
[Mapping of the gene for antidiuretic hormone in Rattus norvegicus].
November 1994, Genetika,
Jeffrey Tseng, and Christy B Erbe, and Anne E Kwitek, and Howard J Jacob, and Paul Popper, and Phillip A Wackym
Histamine H1 receptor down-regulation mediated by M3 muscarinic acetylcholine receptor subtype.
August 2004, Journal of pharmacological sciences,
Copied contents to your clipboard!