Quantification of mesna and total mesna in kidney tissue by high-performance liquid chromatography with electrochemical detection. 2003

Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
Department of Medical Oncology, Vrije Universiteit Medical Center, KRIGO, BR 232, De Boelelaan 1117, 1007 MB, Amsterdam, The Netherlands. m.verschraagen@vumc.nl

A sensitive and selective assay for the determination of mesna and total mesna in tissue was developed and validated. After a simple homogenization, extraction and deproteinization step, mesna could be measured immediately by HPLC with an electrochemical detector provided with a sensitive wall-jet gold electrode. Total mesna (i.e., free mesna and mesna present in mesna disulfides and mixed mesna disulfides) could be measured after pre-column reduction with sodium borohydride to free mesna. The lower limit of quantification of mesna and total mesna was for both compounds 10 nmol/g. The assays for mesna and total mesna in tissue were linear over the ranges of 10-3000 and 10-10000 nmol/g, respectively. The within-day and between-day precisions of both methods were better than 9%. The within-day and between-day accuracy of the mesna assay ranged from 103.7 to 113.6%, whereas the accuracies of the total mesna assay ranged from 97.8 to 106.7%. Mesna in an EDTA containing tissue homogenate or in deproteinized tissue homogenate stored at -80 degrees C was stable for at least 12 weeks. Total mesna was stable under all conditions measured. The developed assays will be applied for the determination of the distribution of mesna and total mesna in tissues of the rat after administration of mesna or BNP7787.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004563 Electrochemistry The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes. Electrochemistries
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D015080 Mesna A sulfhydryl compound used to prevent urothelial toxicity by inactivating metabolites from ANTINEOPLASTIC AGENTS, such as IFOSFAMIDE or CYCLOPHOSPHAMIDE. 2-Mercaptoethanesulfonate,Coenzyme M,Ethanesulfonic acid, 2-mercapto-, monosodium salt,ASTA-D 7093,MESNA-cell,Mesnex,Mesnum,Mistabron,Mistabronco,Mitexan,Mucofluid,Sodium 2-Mercaptoethanesulphonate,UCB-3983,Uromitexan,Ziken,2 Mercaptoethanesulfonate,2-Mercaptoethanesulphonate, Sodium,ASTA D 7093,ASTAD 7093,MESNA cell,UCB 3983,UCB3983
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D020011 Protective Agents Synthetic or natural substances which are given to prevent a disease or disorder or are used in the process of treating a disease or injury due to a poisonous agent. Protective Agent,Protective Drug,Protective Drugs,Agent, Protective,Agents, Protective,Drug, Protective,Drugs, Protective

Related Publications

Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
April 2005, Talanta,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
April 2001, Journal of chromatography. B, Biomedical sciences and applications,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
October 1986, Journal of chromatography,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
February 1991, Journal of chromatography,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
March 1986, Journal of chromatography,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
July 1988, Journal of chromatography,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
September 2007, Journal of chromatography. A,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
February 1997, Journal of chromatography. A,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
June 2001, Journal of chromatography. B, Biomedical sciences and applications,
Miranda Verschraagen, and Marjolein Bosma, and T H Ursula Zwiers, and Emine Torun, and Wim J F van der Vijgh
March 2005, Transplant international : official journal of the European Society for Organ Transplantation,
Copied contents to your clipboard!