BIOMAT Database Logo BIOMAT Database Logo

Enumeration of Legionella CFU by colony hybridization using specific DNA probes. 2002

Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Technology and Development Laboratory, Kurita Water Industries Ltd., Kanagawa 243-0124, Japan. wakako.Satoh@kurita.co.jp

Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.

UI MeSH Term Description Entries
D007875 Legionella Gram-negative aerobic rods, isolated from surface water or thermally polluted lakes or streams. Member are pathogenic for man. Legionella pneumophila is the causative agent for LEGIONNAIRES' DISEASE.
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015169 Colony Count, Microbial Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing. Agar Dilution Count,Colony-Forming Units Assay, Microbial,Fungal Count,Pour Plate Count,Spore Count,Spread Plate Count,Streak Plate Count,Colony Forming Units Assay, Microbial,Colony Forming Units Assays, Microbial,Agar Dilution Counts,Colony Counts, Microbial,Count, Agar Dilution,Count, Fungal,Count, Microbial Colony,Count, Pour Plate,Count, Spore,Count, Spread Plate,Count, Streak Plate,Counts, Agar Dilution,Counts, Fungal,Counts, Microbial Colony,Counts, Pour Plate,Counts, Spore,Counts, Spread Plate,Counts, Streak Plate,Dilution Count, Agar,Dilution Counts, Agar,Fungal Counts,Microbial Colony Count,Microbial Colony Counts,Pour Plate Counts,Spore Counts,Spread Plate Counts,Streak Plate Counts
D015345 Oligonucleotide Probes Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin. Oligodeoxyribonucleotide Probes,Oligonucleotide Probe,Oligoribonucleotide Probes,Probe, Oligonucleotide,Probes, Oligodeoxyribonucleotide,Probes, Oligonucleotide,Probes, Oligoribonucleotide

Related Publications

Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
A rapid procedure for cell colony hybridization using DNA probes.
June 1989, Analytical biochemistry,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Foodborne enterotoxigenic Escherichia coli: detection and enumeration by DNA colony hybridization.
April 1983, Applied and environmental microbiology,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization.
January 1993, Applied and environmental microbiology,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Use of biotinylated DNA probes in colony hybridization.
May 1986, Nucleic acids research,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Enumeration of virulent Yersinia enterocolitica colonies by DNA colony hybridization using alkaline treatment and paper filters.
September 1988, Molecular and cellular probes,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Detection and enumeration of virulent Yersinia enterocolitica in food by DNA colony hybridization.
September 1983, Applied and environmental microbiology,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Enumeration of polysaccharide-degrading Bacteroides species in human feces by using species-specific DNA probes.
February 1986, Applied and environmental microbiology,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Enumeration by DNA colony hybridization of virulent Yersinia enterocolitica colonies in artificially contaminated food.
February 1986, Applied and environmental microbiology,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Enumeration by DNA Colony Hybridization of Virulent Yersinia enterocolitica Colonies in Artificially Contaminated Food.
May 1986, Applied and environmental microbiology,
Wakako Satoh, and Mayumi Nakata, and Hiroyuki Yamamoto, and Takayuki Ezaki, and Keiichi Hiramatsu
Colony hybridization of bacterial isolates with Burkholderia cepacia-specific probes.
January 2002, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!