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Some optical principles of the clinical specular microscope. 1976

W M Bourne, and J M Enoch

The clinical specular microscope is used to examine and photograph human endothelial cells in vivo. An optical analysis is presented here in simple form to aid the ophthalmologist or visual scientist in understanding how this instrument can be used in a quantitative manner to measure the size of the magnified endothelial cells and the thickness of the overlying cornea. An error analysis is discussed in order to demonstrate some aspects of the precision of the determination.

UI MeSH Term Description Entries
D010781 Photography Method of making images on a sensitized surface by exposure to light or other radiant energy. Photographies
D003315 Cornea The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed) Corneas
D004727 Endothelium A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body. Endotheliums
D055095 Optics and Photonics A specialized field of physics and engineering involved in studying the behavior and properties of light and the technology of analyzing, generating, transmitting, and manipulating ELECTROMAGNETIC RADIATION in the visible, infrared, and ultraviolet range. Photonics,Photonics and Optics
D019721 Ophthalmoscopes Devices for examining the interior of the eye, permitting the clear visualization of the structures of the eye at any depth. (UMDNS, 1999) Funduscopes,Funduscope,Ophthalmoscope

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