The caudal homeodomain protein activates Drosophila E2F gene expression. 2002

Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
Department of Molecular Biology, Pusan National University, Pusan 609-735, Korea.

The Drosophila caudal homeobox gene is required for definition of the anteroposterior axis and for gut development, and CDX1 and CDX2, human homologs, play crucial roles in the regulation of cell proliferation and differentiation in the intestine. Most studies have indicated tumor suppressor functions of Cdx2, with inhibition of proliferation, while the effects of Cdx1 are more controversial. The influence of Drosophila Caudal on cell proliferation is unknown. In this study, we found three potential Caudal binding sequences in the 5'-flanking region of the Drosophila E2F (DE2F) gene and showed by transient transfection assays that they are involved in Caudal transactivation of the dE2F gene promoter. Analyses with transgenic flies carrying an E2F-lacZ fusion gene, with and without mutation in the Caudal binding site, indicated that the Caudal binding sites are required for expression of dE2F in living flies. Caudal-induced E2F expression was also confirmed with a GAL4-UAS system in living flies. In addition, ectopic expression of Caudal with heat-shock promotion induced melanotic tumors in larvae. These results suggest that Caudal is involved in regulation of proliferation through transactivation of the E2F gene in Drosophila.

UI MeSH Term Description Entries
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004330 Drosophila A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology. Fruit Fly, Drosophila,Drosophila Fruit Flies,Drosophila Fruit Fly,Drosophilas,Flies, Drosophila Fruit,Fly, Drosophila Fruit,Fruit Flies, Drosophila
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D014157 Transcription Factors Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. Transcription Factor,Factor, Transcription,Factors, Transcription
D015533 Transcriptional Activation Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes. Gene Activation,Genetic Induction,Transactivation,Induction, Genetic,Trans-Activation, Genetic,Transcription Activation,Activation, Gene,Activation, Transcription,Activation, Transcriptional,Genetic Trans-Activation,Trans Activation, Genetic
D015534 Trans-Activators Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins. Nuclear Trans-Acting Factor,Trans-Acting Factors,Trans-Acting Factor,Trans-Activator,Transactivator,Transactivators,Factor, Nuclear Trans-Acting,Factor, Trans-Acting,Factors, Trans-Acting,Nuclear Trans Acting Factor,Trans Acting Factor,Trans Acting Factors,Trans Activator,Trans Activators,Trans-Acting Factor, Nuclear
D050684 E2F Transcription Factors A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation. E2F Transcription Factor,E2F Proteins,Transcription Factor E2F,E2F, Transcription Factor,Factor, E2F Transcription,Transcription Factor, E2F,Transcription Factors, E2F
D018398 Homeodomain Proteins Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL). Homeo Domain Protein,Homeobox Protein,Homeobox Proteins,Homeodomain Protein,Homeoprotein,Homeoproteins,Homeotic Protein,Homeo Domain Proteins,Homeotic Proteins,Domain Protein, Homeo,Protein, Homeo Domain,Protein, Homeobox,Protein, Homeodomain,Protein, Homeotic,Proteins, Homeo Domain,Proteins, Homeobox,Proteins, Homeodomain,Proteins, Homeotic

Related Publications

Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
January 2002, International journal of oncology,
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
November 1996, The Journal of biological chemistry,
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
September 1993, Mechanisms of development,
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
February 1999, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
May 2001, The Journal of biological chemistry,
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
November 1994, Molecular and cellular biology,
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
February 1997, Molecular endocrinology (Baltimore, Md.),
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
April 2009, Brain research,
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
September 2009, Journal of molecular biology,
Mi-Sun Hwang, and Young-Shin Kim, and Na-Hyun Choi, and Jae-Hong Park, and Eun-Jin Oh, and Eun-Jeong Kwon, and Masamitsu Yamaguchi, and Mi-Ae Yoo
January 2014, PloS one,
Copied contents to your clipboard!