Cloning, nucleotide sequencing, and expression of the beta-galactosidase-encoding gene (lacA) from Aspergillus oryzae. 2002

Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
Laboratory of Lactic Acid Bacteria Research, Food Functionality Research Institute, Meiji Dairies Corporation, Odawara 250-0862, Japan. YOSHIYUKI_ITOU@MEIJI-MILK.COM

lacA coding for beta-galactosidase (beta-gal) was cloned from the genomic DNA of Aspergillus oryzae RIB40. There were 9 exons in lacA and the coding region of 3,015 bp encoded a protein of 1,005 aa with a deduced molecular mass of 109,898. A. oryzae lacA was highly homologous to fungal beta-gals, with the highest aa identity of 70.7% to A. niger lacA, and also showed significant identity to acid beta-gals belonging to family 35 glycosyl hydrolases. Approximately 10 copies of lacA under control of A. oryzae glaA promoter were integrated into the chromosome of A. oryzae M-2-3. The recombinant strain expressed more than 700-fold of the beta-gal activity as compared to the wild type strain under induction by maltose.

UI MeSH Term Description Entries
D007438 Introns Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes. Intervening Sequences,Sequences, Intervening,Intervening Sequence,Intron,Sequence, Intervening
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005091 Exons The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA. Mini-Exon,Exon,Mini Exon,Mini-Exons
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001236 Aspergillus oryzae An imperfect fungus present on most agricultural seeds and often responsible for the spoilage of seeds in bulk storage. It is also used in the production of fermented food or drink, especially in Japan. Eurotium oryzae
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D001616 beta-Galactosidase A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1. Lactases,Dairyaid,Lactaid,Lactogest,Lactrase,beta-D-Galactosidase,beta-Galactosidase A1,beta-Galactosidase A2,beta-Galactosidase A3,beta-Galactosidases,lac Z Protein,Protein, lac Z,beta D Galactosidase,beta Galactosidase,beta Galactosidase A1,beta Galactosidase A2,beta Galactosidase A3,beta Galactosidases
D017385 Sequence Homology The degree of similarity between sequences. Studies of AMINO ACID SEQUENCE HOMOLOGY and NUCLEIC ACID SEQUENCE HOMOLOGY provide useful information about the genetic relatedness of genes, gene products, and species. Homologous Sequences,Homologs, Sequence,Sequence Homologs,Homolog, Sequence,Homologies, Sequence,Homologous Sequence,Homology, Sequence,Sequence Homolog,Sequence Homologies,Sequence, Homologous,Sequences, Homologous

Related Publications

Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
August 1995, Bioscience, biotechnology, and biochemistry,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
July 1993, Bioscience, biotechnology, and biochemistry,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
December 2002, Bioscience, biotechnology, and biochemistry,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
December 1991, Gene,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
August 1995, Bioscience, biotechnology, and biochemistry,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
May 1999, Bioscience, biotechnology, and biochemistry,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
September 1991, FEMS microbiology letters,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
October 1995, Bioscience, biotechnology, and biochemistry,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
January 1975, Journal of biochemistry,
Yoshiyuki Ito, and Takashi Sasaki, and Katsuhiko Kitamoto, and Chieko Kumagai, and Kohjiro Takahashi, and Katsuya Gomi, and Gakuzo Tamura
July 2000, Applied microbiology and biotechnology,
Copied contents to your clipboard!