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Embryonic development and pregnancies following sequential culture in human tubal fluid and a modified simplex optimized medium containing amino acids. 2002

Klaus E Wiemer, and Anthony R Anderson, and Melissa L Kyslinger, and Margaret L Weikert
Institute for Assisted Reproduction, Charlotte, NC, USA. kwiemer@carolina.rr.com

Pregnancies following human blastocyst transfers were established using a protein supplemented modified potassium simplex optimized medium containing amino acids (KSOM(AA)). Zygotes were first cultured for 2 days in protein supplemented human tubal fluid medium. Resulting embryos (day 3) were subsequently cultured in protein supplemented KSOM(AA) until day 5 of development. Pregnancy and implantation rates were noted after culture and transfer of blastocysts following this culture scheme. The impact of conventional insemination, intracytoplasmic sperm injection (ICSI) and maternal age on developmental rates as well as pregnancy rates was also evaluated. Maternal age had a significant impact (P < 0.05) on developmental rates of embryos until day 3 of culture. Overall, rate of blastocyst development for cultured day 3 embryos was 62%. Patients >39 years of age had lower (P < 0.05) rates of blastocyst development than patients in the younger subgroups. ICSI had no impact on developmental rates until day 3 of culture or rate of blastocyst development. Ongoing pregnancy and implantation rates following culture in KSOM(AA) were 51 and 37% respectively. These results indicate that KSOM(AA) supports high rate blastocyst development and resulting pregnancy rates.

UI MeSH Term Description Entries
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D001755 Blastocyst A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper. Embryo, Preimplantation,Blastocysts,Embryos, Preimplantation,Preimplantation Embryo,Preimplantation Embryos
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004624 Embryo Transfer The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO. Blastocyst Transfer,Tubal Embryo Transfer,Tubal Embryo Stage Transfer,Embryo Transfers,Transfer, Embryo,Transfers, Embryo
D005260 Female Females
D005314 Embryonic and Fetal Development Morphological and physiological development of EMBRYOS or FETUSES. Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000596 Amino Acids Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins. Amino Acid,Acid, Amino,Acids, Amino
D020554 Sperm Injections, Intracytoplasmic An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE). ICSI,Injections, Sperm, Intracytoplasmic,Intracytoplasmic Sperm Injections,Injection, Intracytoplasmic Sperm,Injections, Intracytoplasmic Sperm,Intracytoplasmic Sperm Injection,Sperm Injection, Intracytoplasmic

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