Spectrophotometric determination of p-aminophenol alone or in the presence of acetaminophen. 1976

E Kalatzis, and I Zarbi

Small amounts of p-aminophenol were analyzed quantitatively by mixing with 4-nitro- or 2,4-dinitrobenzaldehyde in neutral alcoholic solution and then quantitatively forming the respective Schiff bases, 4'-nitro- and 2',4'-dinitrobenzylidene-4-hydroxyaniline, by evaporating the alcoholic solutions to dryness. The Schiff bases thus formed were examined spectrophotometrically. This procedure was also used for the determination of p-aminophenol in the presence of its degradation products. It was necessary, however, to separate the respective Schiff bases by TLC from the degradation products and the reaction mixture prior to spectrophotometric examination. Small amounts of p-aminophenol present in acetaminophen were quantitatively determined by applying the procedure to a water-ethanol (95:5 v/v) extract of the samples of acetaminophen. TLC was used to separate the respective Schiff bases from the reaction mixture and acetaminophen prior to spectrophotometric examination.

UI MeSH Term Description Entries
D002855 Chromatography, Thin Layer Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D004355 Drug Stability The chemical and physical integrity of a pharmaceutical product. Drug Shelf Life,Drugs Shelf Lives,Shelf Life, Drugs,Drug Stabilities,Drugs Shelf Life,Drugs Shelf Live,Life, Drugs Shelf,Shelf Life, Drug,Shelf Live, Drugs,Shelf Lives, Drugs
D000082 Acetaminophen Analgesic antipyretic derivative of acetanilide. It has weak anti-inflammatory properties and is used as a common analgesic, but may cause liver, blood cell, and kidney damage. Acetamidophenol,Hydroxyacetanilide,Paracetamol,APAP,Acamol,Acephen,Acetaco,Acetominophen,Algotropyl,Anacin-3,Datril,N-(4-Hydroxyphenyl)acetanilide,N-Acetyl-p-aminophenol,Panadol,Tylenol,p-Acetamidophenol,p-Hydroxyacetanilide,Anacin 3,Anacin3
D000814 Aniline Compounds Compounds that include the aminobenzene structure. Phenylamine,Phenylamines,Anilines,Compounds, Aniline
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry

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