Recombinant human serotonin 5A receptors stably expressed in C6 glioma cells couple to multiple signal transduction pathways. 2003

Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
Laboratory of Pathophysiology, Kyushu University Graduate School of Pharmaceutical Sciences, Fukuoka, Japan. noda@phar.kyushu-u.ac.jp

Human serotonin 5A (5-HT5A) receptors were stably expressed in undifferentiated C6 glioma. In 5-HT5A receptors-expressing cells, accumulation of cAMP by forskolin was inhibited by 5-HT as reported previously. Pertussis toxin-sensitive inhibition of ADP-ribosyl cyclase was also observed, indicating a decrease of cyclic ADP ribose, a potential intracellular second messenger mediating ryanodine-sensitive Ca2+ mobilization. On the other hand, 5-HT-induced outward currents were observed using the patch-clamp technique in whole-cell configuration. The 5-HT-induced outward current was observed in 84% of the patched 5-HT5A receptor-expressing cells and was concentration-dependent. The 5-HT-induced current was inhibited when intracellular K+ was replaced with Cs+ but was not significantly inhibited by typical K+ channel blockers. The 5-HT-induced current was significantly attenuated by 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) in the patch pipette. Depleting intracellular Ca2+ stores by application of caffeine or thapsigargin also blocked the 5-HT-induced current. Blocking G protein, the inositol triphosphate (IP3) receptor, or pretreatment with pertussis toxin, all inhibited the 5-HT-induced current. IP3 showed a transient increase after application of 5-HT in 5-HT5A receptor-expressing cells. It was concluded that in addition to the inhibition of cAMP accumulation and ADP-ribosyl cyclase activity, 5-HT5A receptors regulate intracellular Ca2+ mobilization which is probably a result of the IP3-sensitive Ca2+ store. These multiple signal transduction systems may induce complex changes in the serotonergic system in brain function.

UI MeSH Term Description Entries
D011985 Receptors, Serotonin Cell-surface proteins that bind SEROTONIN and trigger intracellular changes which influence the behavior of cells. Several types of serotonin receptors have been recognized which differ in their pharmacology, molecular biology, and mode of action. 5-HT Receptor,5-HT Receptors,5-Hydroxytryptamine Receptor,5-Hydroxytryptamine Receptors,Receptors, Tryptamine,Serotonin Receptor,Serotonin Receptors,Tryptamine Receptor,Tryptamine Receptors,Receptors, 5-HT,Receptors, 5-Hydroxytryptamine,5 HT Receptor,5 HT Receptors,5 Hydroxytryptamine Receptor,5 Hydroxytryptamine Receptors,Receptor, 5-HT,Receptor, 5-Hydroxytryptamine,Receptor, Serotonin,Receptor, Tryptamine,Receptors, 5 HT,Receptors, 5 Hydroxytryptamine
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D005910 Glioma Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21) Glial Cell Tumors,Malignant Glioma,Mixed Glioma,Glial Cell Tumor,Glioma, Malignant,Glioma, Mixed,Gliomas,Gliomas, Malignant,Gliomas, Mixed,Malignant Gliomas,Mixed Gliomas,Tumor, Glial Cell,Tumors, Glial Cell
D006493 Heparin A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts. Heparinic Acid,alpha-Heparin,Heparin Sodium,Liquaemin,Sodium Heparin,Unfractionated Heparin,Heparin, Sodium,Heparin, Unfractionated,alpha Heparin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000067956 Adenylyl Cyclase Inhibitors Compounds that bind to and inhibit the action of ADENYLYL CYCLASES. Adenylate Cyclase Inhibitors,Cyclase Inhibitors, Adenylate,Cyclase Inhibitors, Adenylyl,Inhibitors, Adenylate Cyclase,Inhibitors, Adenylyl Cyclase
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012701 Serotonin A biochemical messenger and regulator, synthesized from the essential amino acid L-TRYPTOPHAN. In humans it is found primarily in the central nervous system, gastrointestinal tract, and blood platelets. Serotonin mediates several important physiological functions including neurotransmission, gastrointestinal motility, hemostasis, and cardiovascular integrity. Multiple receptor families (RECEPTORS, SEROTONIN) explain the broad physiological actions and distribution of this biochemical mediator. 5-HT,5-Hydroxytryptamine,3-(2-Aminoethyl)-1H-indol-5-ol,Enteramine,Hippophaine,Hydroxytryptamine,5 Hydroxytryptamine
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured

Related Publications

Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
October 1991, Cell regulation,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
January 2008, Journal of pineal research,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
July 1996, Endocrinology,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
May 1993, Journal of cellular biochemistry,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
June 2000, European journal of pharmacology,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
December 1998, Naunyn-Schmiedeberg's archives of pharmacology,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
June 1994, The Journal of pharmacology and experimental therapeutics,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
August 1996, Endocrinology,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
September 1989, Biochimica et biophysica acta,
Mami Noda, and Satsuki Yasuda, and Mitsuko Okada, and Haruhiro Higashida, and Aki Shimada, and Nakao Iwata, and Norio Ozaki, and Kaori Nishikawa, and Sakiko Shirasawa, and Mayumi Uchida, and Shunsuke Aoki, and Keiji Wada
September 1990, Journal of immunology (Baltimore, Md. : 1950),
Copied contents to your clipboard!