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[Detection of serum anti-B/B' UsnRNP antibodies in patients with connective tissue diseases by immunoblotting]. 2002

A Ghirardello, and A Doria, and S Zampieri, and D Villalta, and R Tozzoli, and L Iaccarino, and R Bendo, and P F Gambari
Cattedra e Divisione di Reumatologia, Dipartimento di Scienze Mediche e Chirurgiche, Università degli Studi di Padova, Italy.

OBJECTIVE To investigate the reliability of the immunoblot method in the detection of serum immunoreactivity towards the B/B' polypeptides of U small nuclear ribonucleoproteins (UsnRNP) and to assess the significance of these antibodies in connective tissue disease (CTD) patients. METHODS We tested the sera of 348 patients with CTD (101 SLE, 51 systemic sclerosis, 53 primary Sjögren's syndrome, 27 poly/dermatomyositis, 15 rheumatoid arthritis and 101 overlap CTD), of 31 matched healthy subjects and 13 patients with primary Epstein-Barr virus (EBV) infection with high titre of IgG anti-EBV antibodies. IgG anti-UsnRNP antibodies were determined by immunoblotting on nuclear extract from Raji cells (an EBV-immortalised human B lymphoid cell line) and Jurkat cells (a human T lymphoid cell line). Anti-dsDNA antibodies were detected by indirect immunofluorescence on Crithidia luciliae and anti-ENA by counterimmunoelectrophoresis. Anti-dsDNA activity and avidity were measured in SLE sera by ELISA with Scatchard analysis. Results were statistically analysed by chi-square and Mann-Whitney tests. RESULTS A high frequency of anti-B/B' antibodies was found in the sera of CTD patients, confined to SLE (54.4%) and overlap CTD with SLE features (55,2%). Anti-B/B' immune reactivity was closely associated with other anti-UsnRNP specificities, gel precipitating anti-nRNP and anti-P antibodies. Nine out of 15 (60%) anti-B/B' positive/anti-ENA negative lupus sera on Raji blots were confirmed to be positive also on Jurkat blots. The sera from patients with EBV infection provided, on Raji blots, completely different band patterns from those obtained with auto-immune sera. CONCLUSIONS. The Sm B/B' proteins are the predominant or, at least, the most frequently targeted antigens of the UsnRNP auto-immune response in SLE and "lupus-like" overlap CTD. Moreover, anti-B/B' is diagnostically specific for CTD with SLE features. Immunoblotting on human B lymphoid cells is a reliable method, in terms of sensitivity and specificity, for the detection of anti-Sm B/B' antibodies.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D003240 Connective Tissue Diseases A heterogeneous group of disorders, some hereditary, others acquired, characterized by abnormal structure or function of one or more of the elements of connective tissue, i.e., collagen, elastin, or the mucopolysaccharides. Connective Tissue Disease,Disease, Connective Tissue,Diseases, Connective Tissue
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001323 Autoantibodies Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them. Autoantibody
D001324 Autoantigens Endogenous tissue constituents with the ability to interact with AUTOANTIBODIES and cause an immune response. Autoantigen,Autologous Antigen,Autologous Antigens,Self-Antigen,Self-Antigens,Antigen, Autologous,Antigens, Autologous,Self Antigen,Self Antigens
D015151 Immunoblotting Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies. Dot Immunoblotting,Electroimmunoblotting,Immunoelectroblotting,Reverse Immunoblotting,Immunoblotting, Dot,Immunoblotting, Reverse,Dot Immunoblottings,Electroimmunoblottings,Immunoblottings,Immunoblottings, Dot,Immunoblottings, Reverse,Immunoelectroblottings,Reverse Immunoblottings
D055517 snRNP Core Proteins The protein components that constitute the common core of small nuclear ribonucleoprotein particles. These proteins are commonly referred as Sm nuclear antigens due to their antigenic nature. Sm Nuclear Antigens,SNRPN,SNRPN Protein,Sm Core Proteins,SmD Antigen,SmD Autoantigen,Small Nuclear Ribonucleoprotein B,Small Nuclear Ribonucleoprotein Core Proteins,Small Nuclear Ribonucleoprotein D1 Polypeptide 16kDa,Small Nuclear Ribonucleoprotein D2 Polypeptide 16.5kDa,Small Nuclear Ribonucleoprotein D3 Polypeptide 18kDa,Small Nuclear Ribonucleoprotein Polypeptide B',Small Nuclear Ribonucleoprotein Polypeptide B'',Small Nuclear Ribonucleoprotein Polypeptide B2,Small Nuclear Ribonucleoprotein Polypeptide E,Small Nuclear Ribonucleoprotein Polypeptide F,Small Nuclear Ribonucleoprotein Sm D1,Small Nuclear Ribonucleoprotein Sm D2,Small Nuclear Ribonucleoprotein-Associated Protein B,Small Nuclear Ribonucleoprotein-Associated Protein B1,Small Nuclear Ribonucleoprotein-Associated Protein B2,Small Nuclear Ribonucleoprotein-Associated Protein D1,Small Nuclear Ribonucleoprotein-Associated Protein D2,Small Nuclear Ribonucleoprotein-Associated Protein D3,Small Nuclear Ribonucleoprotein-Associated Protein E,Small Nuclear Ribonucleoprotein-Associated Protein F,Small Nuclear Ribonucleoprotein-Associated Protein N,snRNP Core Protein D1,Antigen, SmD,Antigens, Sm Nuclear,Autoantigen, SmD,Core Proteins, Sm,Core Proteins, snRNP,Nuclear Antigens, Sm,Small Nuclear Ribonucleoprotein Associated Protein B,Small Nuclear Ribonucleoprotein Associated Protein B1,Small Nuclear Ribonucleoprotein Associated Protein B2,Small Nuclear Ribonucleoprotein Associated Protein D1,Small Nuclear Ribonucleoprotein Associated Protein D2,Small Nuclear Ribonucleoprotein Associated Protein D3,Small Nuclear Ribonucleoprotein Associated Protein E,Small Nuclear Ribonucleoprotein Associated Protein F,Small Nuclear Ribonucleoprotein Associated Protein N

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