Biomaterial Database

[Apoptosis inductive effect of homoharringtonine and isoharringtonine on human acute promyelocytic leukemia HL-60 cells]. 1999

B Shi, and R Han

Department of Pharmacology, Institute of Materia Medica, CAMS and PUMC, Beijing 100050.

OBJECTIVE To study the effects of homoharringtonine and isoharringtonine on the induction of apoptosis on human promyelocytic leukemia HL-60 cells. METHODS By using flow cytometry, DNA gel electrophoresis, microscopy and electron microscopy. RESULTS It was demonstrated that homoharringtonine and isoharringtonine can induce apoptosis of HL-60 cells significantly and rapidly. The activity of homoharringtonine was higher than that of isoharringtonine, and it was time and concentration dependent. CONCLUSIONS Both homoharringtonine and isoharringtonine could induce apoptosis of HL-60 cells in terms of morphology, and DNA ladder assay. The activity of homoharringtonine was much higher than that of isoharringtonine.

UI	MeSH Term	Description	Entries
D006248	Harringtonines	Tetracyclic spiro-BENZAZEPINES isolated from the seeds of CEPHALOTAXUS. They are esters of the alkaloid cephalotaxine and may be effective as antineoplastic agents.	Cephalotaxine Alkaloids,Cephalotaxus Alkaloids,Alkaloids, Cephalotaxine,Alkaloids, Cephalotaxus
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000077863	Homoharringtonine	Semisynthetic derivative of harringtonine that acts as a protein synthesis inhibitor and induces APOPTOSIS in tumor cells. It is used in the treatment of MYELOID LEUKEMIA, CHRONIC.	Ceflatonin,Cephalotaxine,Homoharringtonine (3(R))-isomer,Omacetaxine,Omacetaxine Mepesuccinate,Synribo
D017209	Apoptosis	A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.	Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D018922	HL-60 Cells	A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)	HL60 Cells,Cell, HL60,Cells, HL60,HL 60 Cells,HL-60 Cell,HL60 Cell