Activity blockade increases the number of functional synapses in the hippocampus of newborn rats. 2003

Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
Department of Biosciences, Division of Animal Physiology, P.O. Box 65, University of Helsinki, 00014 Helsinki, Finland.

During development neuronal circuitries are refined by activity. Here we studied the role of spontaneous electrical activity in the regulation of synapse formation in the intact newborn (Postnatal Day 3; P3) rat hippocampus in vitro. The blockade of the spontaneous network activity with TTX led to an increase in the number of functional excitatory synapses in the CA3 area of the developing hippocampus. In parallel, there was a substantial increase in the expression levels of the presynaptic markers synaptophysin, synaptotagmin, and synapsin I and of GluR1 AMPA receptor subunits. These changes were associated with an increase in the frequency and amplitude of AMPA receptor-mediated miniature excitatory postsynaptic currents (mEPSCs). Our correlated immunocytochemical, electronmicroscopical, and electrophysiological experiments indicate that in the developing hippocampus spontaneous network activity controls the number of functional synapses.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D009434 Neural Pathways Neural tracts connecting one part of the nervous system with another. Neural Interconnections,Interconnection, Neural,Interconnections, Neural,Neural Interconnection,Neural Pathway,Pathway, Neural,Pathways, Neural
D009435 Synaptic Transmission The communication from a NEURON to a target (neuron, muscle, or secretory cell) across a SYNAPSE. In chemical synaptic transmission, the presynaptic neuron releases a NEUROTRANSMITTER that diffuses across the synaptic cleft and binds to specific synaptic receptors, activating them. The activated receptors modulate specific ion channels and/or second-messenger systems in the postsynaptic cell. In electrical synaptic transmission, electrical signals are communicated as an ionic current flow across ELECTRICAL SYNAPSES. Neural Transmission,Neurotransmission,Transmission, Neural,Transmission, Synaptic
D009473 Neuronal Plasticity The capacity of the NERVOUS SYSTEM to change its reactivity as the result of successive activations. Brain Plasticity,Plasticity, Neuronal,Axon Pruning,Axonal Pruning,Dendrite Arborization,Dendrite Pruning,Dendritic Arborization,Dendritic Pruning,Dendritic Remodeling,Neural Plasticity,Neurite Pruning,Neuronal Arborization,Neuronal Network Remodeling,Neuronal Pruning,Neuronal Remodeling,Neuroplasticity,Synaptic Plasticity,Synaptic Pruning,Arborization, Dendrite,Arborization, Dendritic,Arborization, Neuronal,Arborizations, Dendrite,Arborizations, Dendritic,Arborizations, Neuronal,Axon Prunings,Axonal Prunings,Brain Plasticities,Dendrite Arborizations,Dendrite Prunings,Dendritic Arborizations,Dendritic Prunings,Dendritic Remodelings,Network Remodeling, Neuronal,Network Remodelings, Neuronal,Neural Plasticities,Neurite Prunings,Neuronal Arborizations,Neuronal Network Remodelings,Neuronal Plasticities,Neuronal Prunings,Neuronal Remodelings,Neuroplasticities,Plasticities, Brain,Plasticities, Neural,Plasticities, Neuronal,Plasticities, Synaptic,Plasticity, Brain,Plasticity, Neural,Plasticity, Synaptic,Pruning, Axon,Pruning, Axonal,Pruning, Dendrite,Pruning, Dendritic,Pruning, Neurite,Pruning, Neuronal,Pruning, Synaptic,Prunings, Axon,Prunings, Axonal,Prunings, Dendrite,Prunings, Dendritic,Prunings, Neurite,Prunings, Neuronal,Prunings, Synaptic,Remodeling, Dendritic,Remodeling, Neuronal,Remodeling, Neuronal Network,Remodelings, Dendritic,Remodelings, Neuronal,Remodelings, Neuronal Network,Synaptic Plasticities,Synaptic Prunings
D009924 Organ Culture Techniques A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1) Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D002135 Calcium-Binding Proteins Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS. Calcium Binding Protein,Calcium-Binding Protein,Calcium Binding Proteins,Binding Protein, Calcium,Binding Proteins, Calcium,Protein, Calcium Binding,Protein, Calcium-Binding
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell

Related Publications

Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
January 2012, Frontiers in neuroanatomy,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
August 2007, The Journal of physiology,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
November 2008, The Journal of physiology,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
January 1989, Zeitschrift fur mikroskopisch-anatomische Forschung,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
November 2007, The European journal of neuroscience,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
January 2013, PloS one,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
November 2019, Behavioural brain research,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
January 2004, The Journal of physiology,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
August 2004, The Journal of physiology,
Sari E Lauri, and Karri Lamsa, and Ivan Pavlov, and Ruusu Riekki, and Benjamin E Johnson, and Elek Molnar, and Heikki Rauvala, and Tomi Taira
January 2007, Hippocampus,
Copied contents to your clipboard!