Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom. 2003

Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
Department of Animal Toxicology, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China.

An L-amino acid oxidase (TM-LAO) from the venom of Hunan Trimeresurus mucrosquamatus was purified to homogeneity by three steps including DEAE Sephadex A-50 ion-exchange chromatography, Sephadex G-75 gel filtration and Resource Q ion-exchange chromatography. TM-LAO is composed of two identical subunits with a molecular weight of 55 kD by SDS-polyacrylamide gel electrophoresis. The molecular weight was different with that of LAO purified from the same species distributed in Taiwan that was 70 kD. The 24 N-terminal amino acid sequence of TM-LAO is ADNKNPLEECFRETNYEEFLEIAR, which shares high similarity with other Viperid snake venom LAOs and has moderate similarity with Elapid snake venom LAOs. Further studies found that TM-LAO inhibited the growth of E. coli, S. aurues and B. dysenteriae. TM-LAO also showed cytotoxicity and platelet aggregation activity. All the biological activities were eliminated by catalase, a H(2)O(2) scavenger. It was shown that these biological effects were possibly due to the formation of H(2)O(2) produced by TM-LAO.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D010974 Platelet Aggregation The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS. Aggregation, Platelet
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002461 Cell Line, Transformed Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals. Transformed Cell Line,Cell Lines, Transformed,Transformed Cell Lines
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002852 Chromatography, Ion Exchange Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D003435 Crotalid Venoms Venoms from snakes of the subfamily Crotalinae or pit vipers, found mostly in the Americas. They include the rattlesnake, cottonmouth, fer-de-lance, bushmaster, and American copperhead. Their venoms contain nontoxic proteins, cardio-, hemo-, cyto-, and neurotoxins, and many enzymes, especially phospholipases A. Many of the toxins have been characterized. Bothrops Venom,Crotalidae Venoms,Pit Viper Venoms,Rattlesnake Venoms,Crotactin,Crotalid Venom,Crotalin,Crotaline Snake Venom,Crotalotoxin,Crotamin,Pit Viper Venom,Rattlesnake Venom,Snake Venom, Crotaline,Venom, Bothrops,Venom, Crotalid,Venom, Crotaline Snake,Venom, Pit Viper,Venom, Rattlesnake,Venoms, Crotalid,Venoms, Crotalidae,Venoms, Pit Viper,Venoms, Rattlesnake,Viper Venom, Pit
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004487 Edema Abnormal fluid accumulation in TISSUES or body cavities. Most cases of edema are present under the SKIN in SUBCUTANEOUS TISSUE. Dropsy,Hydrops,Anasarca

Related Publications

Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
January 1988, Toxicon : official journal of the International Society on Toxinology,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
January 1986, The International journal of biochemistry,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
December 1986, Gaoxiong yi xue ke xue za zhi = The Kaohsiung journal of medical sciences,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
July 1978, Biochimica et biophysica acta,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
September 2003, Biochemical and biophysical research communications,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
May 2002, Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
February 2008, Toxicon : official journal of the International Society on Toxinology,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
May 1982, Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
December 1973, Comparative biochemistry and physiology. B, Comparative biochemistry,
Ji-Fu Wei, and Qin Wei, and Qiu-Min Lu, and Hong Tai, and Yang Jin, and Wan-Yu Wang, and Yu-Liang Xiong
November 2009, Toxicon : official journal of the International Society on Toxinology,
Copied contents to your clipboard!