BIOMAT Database Logo BIOMAT Database Logo

The dihydroceramide desaturase is not essential for cell viability in Schizosaccharomyces pombe. 2003

Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
Long Ashton Research Station, Long Ashton, Bristol BS41 9AF, UK.

Recent studies have identified a new family of desaturase-like polypeptide sequences in many higher eukaryotes. Functional characterisation of one member of this family, from Schizosaccharomyces pombe, revealed the enzyme to be a sphingolipid desaturase. This S. pombe gene designated SDCB3b8.07c was identified as the dihydroceramide Delta(4)-desaturase, responsible for the synthesis of sphingosine. Homologous recombination was used to disrupt the endogenous S. pombe dihydroceramide Delta(4)-desaturase. Surprisingly, this had no effect on cell viability, indicating that sphingosine may not be crucial for normal S. pombe functions. This observation has implications for our understanding of the role of sphingosine and its phosphorylated metabolite sphingosine-1-phosphate in lower eukaryotes.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010088 Oxidoreductases The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9) Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012568 Schizosaccharomyces A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales. Fission Yeast,Schizosaccharomyces malidevorans,Schizosaccharomyces pombe,Yeast, Fission,S pombe,Fission Yeasts
D016366 Open Reading Frames A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR). ORFs,Protein Coding Region,Small Open Reading Frame,Small Open Reading Frames,sORF,Unassigned Reading Frame,Unassigned Reading Frames,Unidentified Reading Frame,Coding Region, Protein,Frame, Unidentified Reading,ORF,Open Reading Frame,Protein Coding Regions,Reading Frame, Open,Reading Frame, Unassigned,Reading Frame, Unidentified,Region, Protein Coding,Unidentified Reading Frames
D017386 Sequence Homology, Amino Acid The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species. Homologous Sequences, Amino Acid,Amino Acid Sequence Homology,Homologs, Amino Acid Sequence,Homologs, Protein Sequence,Homology, Protein Sequence,Protein Sequence Homologs,Protein Sequence Homology,Sequence Homology, Protein,Homolog, Protein Sequence,Homologies, Protein Sequence,Protein Sequence Homolog,Protein Sequence Homologies,Sequence Homolog, Protein,Sequence Homologies, Protein,Sequence Homologs, Protein
D017931 DNA Primers Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques. DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide

Related Publications

Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
The Schizosaccharomyces pombe homologue of the chaperone calnexin is essential for viability.
March 1995, The Journal of biological chemistry,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
DNA polymerase 5 acetylation by Eso1 is essential for Schizosaccharomyces pombe viability.
December 2017, International journal of molecular medicine,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
The RNA components of Schizosaccharomyces pombe RNase P are essential for cell viability.
January 1987, Gene,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
Regulation of cell wall synthesis by the clathrin light chain is essential for viability in Schizosaccharomyces pombe.
January 2013, PloS one,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
β(1,3)-glucanosyl-transferase activity is essential for cell wall integrity and viability of Schizosaccharomyces pombe.
November 2010, PloS one,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe.
February 1999, Journal of bacteriology,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
The multiprotein exocyst complex is essential for cell separation in Schizosaccharomyces pombe.
February 2002, Molecular biology of the cell,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
Schizosaccharomyces pombe Noc3 is essential for ribosome biogenesis and cell division but not DNA replication.
September 2008, Eukaryotic cell,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
The beta-1,3-glucanosyltransferase gas4p is essential for ascospore wall maturation and spore viability in Schizosaccharomyces pombe.
June 2008, Molecular microbiology,
Sarah Garton, and Louise V Michaelson, and Frédéric Beaudoin, and Michael H Beale, and Johnathan A Napier
An Atg10-like E2 enzyme is essential for cell cycle progression but not autophagy in Schizosaccharomyces pombe.
January 2013, Cell cycle (Georgetown, Tex.),
Copied contents to your clipboard!