[Effects of polyamine-loaded liposomes on liver mitochondrial permeability transition]. 2002

M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
Facultatea de Medicină Stomatologică, Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi.

The objective of the present study was represented by the effects of polyamineloaded liposomes on hepatic mitochondrial permeability transition (MPT). MPT was appreciated through the swelling of the isolated guinea-pig liver mithocondria induced by Ca2+, at 540 nm, using a diode-array 8452 UV-VIS spectrophotometer and a General Purpose software (Hewlett-Packard). Polyamines encapsulated in liposomes might inhibit the appearance of MPT induced by Ca2+, directly proportional to electrical charge: spermine > spermidine > putrescine > cadaverine. Thus, together with some previous data, it was observed that spermine may modulate hepatic MPT from the exterior, as well as from the interior of mitochondria. Moreover, spermidine and putrescine may also modulate MPT from the interior of mitochondria at low doses, and from the exterior only at high doses. The changes in mitochondrial membranes lipid composition (as done by control liposomes) are slightly influencing the MPT. The increase in membrane rigidity through the use of 40% cholesterol-enriched liposomes is drastically decreasing the appearance and development of MPT.

UI MeSH Term Description Entries
D008081 Liposomes Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins. Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D008930 Mitochondria, Liver Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4) Liver Mitochondria,Liver Mitochondrion,Mitochondrion, Liver
D011700 Putrescine A toxic diamine formed by putrefaction from the decarboxylation of arginine and ornithine. 1,4-Butanediamine,1,4-Diaminobutane,Tetramethylenediamine,1,4 Butanediamine,1,4 Diaminobutane
D002463 Cell Membrane Permeability A quality of cell membranes which permits the passage of solvents and solutes into and out of cells. Permeability, Cell Membrane
D003196 Computer Graphics The process of pictorial communication, between human and computers, in which the computer input and output have the form of charts, drawings, or other appropriate pictorial representation. Computer Graphic,Graphic, Computer,Graphics, Computer
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013053 Spectrophotometry The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D013096 Spermine A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at all pH values. Spermine is associated with nucleic acids, particularly in viruses, and is thought to stabilize the helical structure.
D015317 Biogenic Polyamines Biogenic amines having more than one amine group. These are long-chain aliphatic compounds that contain multiple amino and/or imino groups. Because of the linear arrangement of positive charge on these molecules, polyamines bind electrostatically to ribosomes, DNA, and RNA. Polyamines, Biogenic

Related Publications

M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
April 2011, Journal of bioenergetics and biomembranes,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
April 1996, Biochemistry and molecular biology international,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
January 2000, Toxicology letters,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
June 2020, Toxicology mechanisms and methods,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
July 2004, Biochimica et biophysica acta,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
November 2010, Neurochemical research,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
November 2022, Cells,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
February 2002, Nitric oxide : biology and chemistry,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
April 2004, Free radical biology & medicine,
M Costuleanu, and Liliana Foia, and Natalia Costuleanu, and Anca Indrei, and Simona Mihaela Slătineanu, and Gh Petrescu
May 1995, Archives of biochemistry and biophysics,
Copied contents to your clipboard!