Flow cytometric assessment of ethylene glycol monoethyl ether on spermatogenesis in rats. 2003

Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
Department of Pathology, National Institute of Toxicological Research, Korea Food & Drug Administration, Seoul, Japan.

The effects of ethylene glycol monoethyl ether (EGEE) on testicular cell populations in rats were investigated by a flow cytometric method. Rats were administered by gavage with EGEE at the various doses of 0 (saline alone), 100, 200, 400, and 800 mg/kg body weight/day for 4 weeks. The treatment of EGEE caused decreases in the weight of testis and epididymis and in the number of testicular cells. Histopathologically, exfoliation of germ cells into the tubular lumen was observed at the doses of above 200 mg/kg. The treatment of EGEE at the dose of 400 mg/kg caused moderate testicular degeneration. A significant depletion of haploid cells and a disproportionate ratio of diploid and tetraploid cells were observed as determined by flow cytometric analysis. These results indicate that the toxic effect of EGEE on the male reproductive system may be strongly associated with the disproportion of testicular germ cells.

UI MeSH Term Description Entries
D008297 Male Males
D011003 Ploidies The degree of replication of the chromosome set in the karyotype. Ploidy
D001835 Body Weight The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms. Body Weights,Weight, Body,Weights, Body
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005026 Ethylene Glycols An ethylene compound with two hydroxy groups (-OH) located on adjacent carbons. They are viscous and colorless liquids. Some are used as anesthetics or hypnotics. However, the class is best known for their use as a coolant or antifreeze. Dihydroxyethanes,Ethanediols,Glycols, Ethylene
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013091 Spermatogenesis The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA. Spermatocytogenesis,Spermiogenesis
D013094 Spermatozoa Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility. Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D013737 Testis The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS. Testicles,Testes,Testicle

Related Publications

Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
August 1984, Environmental health perspectives,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
February 1997, Environmental health perspectives,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
January 1992, Scandinavian journal of work, environment & health,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
August 1984, Environmental health perspectives,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
February 1997, Environmental health perspectives,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
October 2006, Industrial health,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
January 1965, American Industrial Hygiene Association journal,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
August 2007, Industrial health,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
April 2013, Andrologia,
Chang Yong Yoon, and Choong Man Hong, and Yong-Yeon Cho, and Yong Hyun Chung, and Hong Ki Min, and Young Won Yun, and Beom Jun Lee, and Ki Hwa Yang, and Yong Soon Lee, and Cheol Kyu Kim
May 1992, MMWR. Morbidity and mortality weekly report,
Copied contents to your clipboard!