Transplantation of autologous endothelial cells induces angiogenesis. 2003

Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
Heart Care Associates and Milwaukee Heart Institute, Milwaukee, Wisconsin, USA.

This study examined the feasibility and efficacy of autologous endothelial cell (EC) transplantation using a fibrin matrix in the ischemic myocardium of sheep. Four weeks after placing an ameroid constrictor in the circumflex artery of 12 adult sheep, four animals (EC group) were subjected to EC transplantation. In four others (saline [SAL] group) saline with added inactivated cells was injected and four animals served as controls. Eight weeks after treatment the animals were sacrificed to assess histology and ultrastructure. Eight weeks after injection, ventricular function was markedly improved in the EC transplant group, but had deteriorated in the SAL and control groups. Myocardial blood flow was also increased in the EC group. Histology and electron microscopy revealed extensive neovascularization after EC transplantation and improved myocardial appearance. Heterotopic transplantation of EC within a fibrin matrix enhances neovascularization, increases myocardial blood flow, and improves left ventricular function.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007267 Injections Introduction of substances into the body using a needle and syringe. Injectables,Injectable,Injection
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009206 Myocardium The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow. Muscle, Cardiac,Muscle, Heart,Cardiac Muscle,Myocardia,Cardiac Muscles,Heart Muscle,Heart Muscles,Muscles, Cardiac,Muscles, Heart
D003326 Coronary Circulation The circulation of blood through the CORONARY VESSELS of the HEART. Circulation, Coronary
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D005340 Fibrinogen Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products. Coagulation Factor I,Factor I,Blood Coagulation Factor I,gamma-Fibrinogen,Factor I, Coagulation,gamma Fibrinogen
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D013917 Thrombin An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN. Thrombase,Thrombin JMI,Thrombin-JMI,Thrombinar,Thrombostat,alpha-Thrombin,beta,gamma-Thrombin,beta-Thrombin,gamma-Thrombin,JMI, Thrombin

Related Publications

Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
October 2004, Current opinion in biotechnology,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
March 1999, The Journal of biological chemistry,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
August 2007, Transplant international : official journal of the European Society for Organ Transplantation,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
September 2008, Journal of neuroscience research,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
November 2008, International journal of stem cells,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
December 2003, International angiology : a journal of the International Union of Angiology,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
September 2013, Molecular and cellular biochemistry,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
August 2004, Photodermatology, photoimmunology & photomedicine,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
November 2001, The Journal of thoracic and cardiovascular surgery,
Valeri Chekanov, and Massod Akhtar, and Guennady Tchekanov, and George Dangas, and Muhammad Z Shehzad, and Fermin Tio, and Milena Adamian, and Antonio Colombo, and Gary Roubin, and Martin B Leon, and Jeffrey W Moses, and Nicholas N Kipshidze
September 2014, Investigative ophthalmology & visual science,
Copied contents to your clipboard!