BIOMAT Database Logo BIOMAT Database Logo

A strategy for mapping the heterochromatin of chromosome 2 of Drosophila melanogaster. 2003

Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Department of Zoology, University of Washington, Box 351800, Seattle, WA 98195, USA.

The heterochromatin of chromosome 2 of Drosophila melanogaster has been among the best characterized models for functional studies of heterochromatin owing to its abundance of genetic markers. To determine whether it might also provide a favorable system for mapping extended regions of heterochromatin, we undertook a project to molecularly map the heterochromatin of the left arm of chromosome 2 (2Lh). In this paper, we describe a strategy that used clones and sequence information available from the Drosophila Genome Project and chromosome rearrangements to construct a map of the distal most portion of 2Lh. We also describe studies that used fluorescent in situ hybridization (FISH) to examine the resolution of this technique for cytologically resolving heterochromatic sequences on mitotic chromosomes. We discuss how these mapping studies can be extended to more proximal regions of the heterochromatin to determine the structural patterns and physical dimensions of 2Lh and the relationship of structure to function.

UI MeSH Term Description Entries
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D004331 Drosophila melanogaster A species of fruit fly frequently used in genetics because of the large size of its chromosomes. D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D006570 Heterochromatin The portion of chromosome material that remains condensed and is transcriptionally inactive during INTERPHASE. Heterochromatins
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015139 Blotting, Southern A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Southern Blotting,Blot, Southern,Southern Blot
D015183 Restriction Mapping Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA. Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction
D017404 In Situ Hybridization, Fluorescence A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei. FISH Technique,Fluorescent in Situ Hybridization,Hybridization in Situ, Fluorescence,FISH Technic,Hybridization in Situ, Fluorescent,In Situ Hybridization, Fluorescent,FISH Technics,FISH Techniques,Technic, FISH,Technics, FISH,Technique, FISH,Techniques, FISH
D022202 Chromosomes, Artificial, Bacterial DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes. Artificial Chromosomes, Bacterial,Bacterial Artificial Chromosomes,BAC (Chromosome),BACs (Chromosomes),Chromosomes, Bacterial Artificial,Artificial Chromosome, Bacterial,Bacterial Artificial Chromosome,Chromosome, Bacterial Artificial
D030561 Databases, Nucleic Acid Databases containing information about NUCLEIC ACIDS such as BASE SEQUENCE; SNPS; NUCLEIC ACID CONFORMATION; and other properties. Information about the DNA fragments kept in a GENE LIBRARY or GENOMIC LIBRARY is often maintained in DNA databases. DDBJ,DNA Data Bank of Japan,DNA Data Banks,DNA Databases,Databases, DNA,Databases, DNA Sequence,Databases, Nucleic Acid Sequence,Databases, RNA,Databases, RNA Sequence,EMBL Nucleotide Sequence Database,GenBank,Nucleic Acid Databases,RNA Databases,DNA Databanks,DNA Sequence Databases,European Molecular Biology Laboratory Nucleotide Sequence Database,Nucleic Acid Sequence Databases,RNA Sequence Databases,Bank, DNA Data,Banks, DNA Data,DNA Data Bank,DNA Databank,DNA Database,DNA Sequence Database,Data Bank, DNA,Data Banks, DNA,Databank, DNA,Databanks, DNA,Database, DNA,Database, DNA Sequence,Database, Nucleic Acid,Database, RNA,Database, RNA Sequence,Nucleic Acid Database,RNA Database,RNA Sequence Database,Sequence Database, DNA,Sequence Database, RNA,Sequence Databases, DNA,Sequence Databases, RNA

Related Publications

Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Alpha and beta heterochromatin in polytene chromosome 2 of Drosophila melanogaster.
December 1996, Chromosoma,
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
June 2007, Science (New York, N.Y.),
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
A Distinct type of heterochromatin within Drosophila melanogaster chromosome 4.
March 2007, Genetics,
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Mapping the pericentric heterochromatin by comparative genomic hybridization analysis and chromosome deletions in Drosophila melanogaster.
December 2012, Genome research,
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Cytogenetic analysis of the second chromosome heterochromatin of Drosophila melanogaster.
March 1991, Genetics,
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Cytogenetic analysis of the third chromosome heterochromatin of Drosophila melanogaster.
February 2002, Genetics,
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Analyzing heterochromatin formation using chromosome 4 of Drosophila melanogaster.
January 2004, Cold Spring Harbor symposia on quantitative biology,
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
On biological functions mapping to the heterochromatin of Drosophila melanogaster.
April 1985, Genetics,
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Genetic analysis of the centromeric heterochromatin of chromosome 2 of Drosophila melanogaster: deficiency mapping of EMS-induced lethal complementation groups.
August 1976, Genetics,
Jiro C Yasuhara, and Marcella Marchetti, and Laura Fanti, and Sergio Pimpinelli, and Barbara T Wakimoto
Genetic analysis of the second chromosome centromeric heterochromatin of Drosophila melanogaster.
June 2003, Genome,
Copied contents to your clipboard!