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OBJECTIVE To investigate the microtensile bond strength (microTBS) of a self-etching priming adhesive system to normal, caries-affected and caries-infected dentin, and to observe the ultrastructure of the resin-dentin interface by transmission electron microscopy (TEM). METHODS Twelve extracted human molar teeth with deep occlusal caries were stained with caries detector solution and ground flat occlusally. The red-stained soft dentin was classified as caries-infected. The surrounding discolored dentin was classified as caries-affected dentin. The surrounding normal dentin served as a control. The entire flat surface was bonded with Clearfil Liner Bond 2V (CV) and covered with resin composite to form a composite crown 5 mm high. One day later the specimens were serially sectioned vertically into multiple slabs 0.8 mm thick. Under microscopic observation, the specimens were divided into normal or caries-infected or caries-affected dentin. These regions were isolated by cutting away the remaining dentin to form hour-glass shapes with the smallest surface area at the test site. After measuring the areas, the specimens were fixed to a microtensile tester and pulled under tension to failure. Additional slabs that were not used for bond strength tests were processed for TEM. Bond strength data were analyzed by Kruskal-Wallis one-way ANOVA and Student-Newman-Keuls multiple comparisons. RESULTS The microTBS of CV to normal, caries-affected and caries-infected dentin were 45 +/- 10 MPa, 30 +/- 10 MPa, 10 +/- 5 MPa, respectively. TEM images showed that CV formed thin hybrid layers that were less than 1 microm thick in normal dentin, but that were between 6-8 microm thick in caries-affected dentin. Bacteria were only sparsely observed in the dentin tubules of bonded caries-affected dentin. However, in caries-infected dentin, an unusual interface was seen in which carious bacteria within disorganized non-banded collagen fibrils could be seen embedded by the adhesive. The hybrid layer in caries-infected dentin was found to be 30-60 microm thick.
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
January 2005,
The journal of adhesive dentistry,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
December 1981,
Kokubyo Gakkai zasshi. The Journal of the Stomatological Society, Japan,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
January 2001,
The International journal of prosthodontics,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
June 2003,
Journal of the California Dental Association,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
December 1988,
Scandinavian journal of dental research,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
July 2008,
Dental materials : official publication of the Academy of Dental Materials,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
September 1984,
Shika zairyo, kikai = Journal of the Japanese Society for Dental Materials and Devices,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
February 1997,
Endodontics & dental traumatology,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
January 2016,
Dental materials journal,
Masahiro Yoshiyama, and
Franklin R Tay, and
Yasuhiro Torii, and
Yoshihiro Nishitani, and
Junichi Doi, and
Kousuke Itou, and
Bernard Ciucchi, and
David H Pashley
January 2002,
Operative dentistry,
