BIOMAT Database Logo BIOMAT Database Logo

[Preparative isolation and study of the reassociation kinetics of a T2 phage DNA fraction containing readily melting regions]. 1976

A V Shugaliĭ, and A B Fonarev

A DNA fraction comprising 6% of total DNA and containing readily-melting regions is isolated from phage T2 DNA using preparative chromatography on MAK columns at T congruent to T m--3 degrees C. Two denaturation regions, differing in the stability for 7 degrees, were observed on this DNA melting curve. A sharp increase of the reassociation rate at initial moments under reassociation temperatures T r approximately less than m --25 degrees C was observed. Thermodynamic characteristics obtained under the repeated melting of DNA fragments after reassociation confirm the fact, that under these reassociation temperatures the incorporation of readily-melting regions into spiral duplexes takes place.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D002621 Chemistry A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D003090 Coliphages Viruses whose host is Escherichia coli. Escherichia coli Phages,Coliphage,Escherichia coli Phage,Phage, Escherichia coli,Phages, Escherichia coli
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D013816 Thermodynamics A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed) Thermodynamic
D055598 Chemical Phenomena The composition, structure, conformation, and properties of atoms and molecules, and their reaction and interaction processes. Chemical Concepts,Chemical Processes,Physical Chemistry Concepts,Physical Chemistry Processes,Physicochemical Concepts,Physicochemical Phenomena,Physicochemical Processes,Chemical Phenomenon,Chemical Process,Physical Chemistry Phenomena,Physical Chemistry Process,Physicochemical Phenomenon,Physicochemical Process,Chemical Concept,Chemistry Process, Physical,Chemistry Processes, Physical,Concept, Chemical,Concept, Physical Chemistry,Concept, Physicochemical,Concepts, Chemical,Concepts, Physical Chemistry,Concepts, Physicochemical,Phenomena, Chemical,Phenomena, Physical Chemistry,Phenomena, Physicochemical,Phenomenon, Chemical,Phenomenon, Physicochemical,Physical Chemistry Concept,Physicochemical Concept,Process, Chemical,Process, Physical Chemistry,Process, Physicochemical,Processes, Chemical,Processes, Physical Chemistry,Processes, Physicochemical

Related Publications

A V Shugaliĭ, and A B Fonarev
[Structural characteristics (reassociation and melting kinetics) of kinetoplast DNA from Crithidia oncopelti].
August 1977, Biokhimiia (Moscow, Russia),
A V Shugaliĭ, and A B Fonarev
Detection of low-melting sites in the phage T2 DNA molecule.
January 1971, Molecular biology,
A V Shugaliĭ, and A B Fonarev
Localization of low-melting regions in phage T7 DNA.
April 1983, Nucleic acids research,
A V Shugaliĭ, and A B Fonarev
Unwinding kinetics of cooperatively melting regions in DNA.
March 1984, Biopolymers,
A V Shugaliĭ, and A B Fonarev
Study of low-melting segments in T2 phage DNA by electron microscopy and the kinetic formaldehyde method.
January 1972, Molecular biology,
A V Shugaliĭ, and A B Fonarev
[Application of DNA sonication in the study of reassociation kinetics].
January 1975, Biokhimiia (Moscow, Russia),
A V Shugaliĭ, and A B Fonarev
Reassociation kinetics of polyploid hepatocyte DNA.
January 1977, Biochimica et biophysica acta,
A V Shugaliĭ, and A B Fonarev
Comparative DNA reassociation kinetics of cranes.
April 1989, Biochemical genetics,
A V Shugaliĭ, and A B Fonarev
Fluorometric measurement of DNA reassociation kinetics.
April 1979, Analytical biochemistry,
A V Shugaliĭ, and A B Fonarev
[Effect of single-stranded and double-stranded breaks on the melting temperature of phage T2 DNA].
January 1979, Molekuliarnaia biologiia,
Copied contents to your clipboard!