A patient-isolated calibration system is described for use with catheter transducer physiological pressure measuring apparatus. A subatmospheric calibration pressure is applied to the rear of the transducer diaphragm, the latter isolating the patient hydraulic circuit from the calibration system. The calibration system need not therefore be sterile and remains assembled to the transducer table. Risk of air embolus in the patient and of over-pressurising the transducer during calibration are minimised. Simplification of the hydraulic circuit reduces the chance of air entrapment, improving frequency response and reproducability of results and preparation time is greatly reduced. The system is easy to make and has proved simple, rugged and reliable in use.