Spectrophotometric assay of yeast vitality using 2,3,5,6-tetramethyl-1,4-benzoquinone and tetrazolium salts. 2003

Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume 839-0861, Japan. tukatani@fitc.pref.fukuoka.jp

A method for the spectrophotometric assay of yeast vitality was developed using 2,3,5,6-tetramethyl-1,4-benzoquinone and tetrazolium salts. The metabolic efficiency of 2,3,5,6-tetramethyl-1,4-benzoquinone by yeast cells was used as an index of yeast vitality. 2,3,5,6-Tetramethyl-1,4-benzoquinone was reduced to 2,3,5,6-tetramethyl-1,4-hydroquinone by yeast cells. Then, the superoxide anion radicals generated from O2 by reduction with 2,3,5,6-tetramethyl-1,4-hydroquinone under alkaline conditions reduced tetrazolium salts to formazan, which exhibited absorbance maxima at 440 nm. A linear relationship between the absorbance and viable cell density was obtained in the range of 1.0 x 10(5)-2.0 x 10(7) cells/ml for a sample solution. During the cultivation of yeast cells, the absorbance showed almost an anti-parallel change with that of glucose in yeast growth and fermentation, suggesting that the absorbance change reflected the vitality of yeast cells.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D004578 Electron Spin Resonance Spectroscopy A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING. ENDOR,Electron Nuclear Double Resonance,Electron Paramagnetic Resonance,Paramagnetic Resonance,Electron Spin Resonance,Paramagnetic Resonance, Electron,Resonance, Electron Paramagnetic,Resonance, Electron Spin,Resonance, Paramagnetic
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry
D013778 Tetrazolium Salts Quaternary salts derived from tetrazoles. They are used in tests to distinguish between reducing sugars and simple aldehydes, for detection of dehydrogenase in tissues, cells, and bacteria, for determination of corticosteroids, and in color photography. (From Mall's Dictionary of Chemistry, 5th ed, p455) Tetrazolium Salt,Salt, Tetrazolium,Salts, Tetrazolium
D016227 Benzoquinones Benzene rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups. 1,2-Benzoquinones,1,4-Benzoquinones,Benzodiones,2,5-Cyclohexadiene-1,4-Diones,o-Benzoquinones,p-Benzoquinones

Related Publications

Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
December 1976, Analytical chemistry,
Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
June 2010, International journal of biomedical science : IJBS,
Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
October 2001, Acta crystallographica. Section C, Crystal structure communications,
Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
March 2020, Chemical science,
Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
October 2002, Journal of pharmaceutical and biomedical analysis,
Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
July 2002, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
December 2006, Analytical biochemistry,
Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
January 2023, Chemical communications (Cambridge, England),
Tadayuki Tsukatani, and Takahiro Oba, and Hiroyuki Ukeda, and Kiyoshi Matsumoto
January 1999, Bioscience, biotechnology, and biochemistry,
Copied contents to your clipboard!